Induction of PR-10 genes and metabolites in strawberry plants in response to Verticillium dahliae infection
Induction of PR-10 genes and metabolites in strawberry plants in response to Verticillium dahliae infection
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DOI：
10.1186/s12870-019-1718-x
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发表时间：
2019-04-05
影响因子：
5.3
通讯作者：
Schwab, Wilfried
中科院分区：
文献类型：
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作者：
Besbes, Fatma;Habegger, Ruth;Schwab, Wilfried
背景大丽轮枝菌是一种土传维管病原菌，可引起草莓等多种植物严重的枯萎病。为加深对大丽轮枝菌对F. x ananassa中，通过qPCR分析研究了21个PR-10基因的表达模式，并通过LC-MS测定了体外F.病原体infection.ResultsThe表达模式的21种异构体表现出广泛的反应。4个PR-10基因在病原菌感染后在叶片中被高度诱导，而8个成员在根中显著上调。5个PR-10基因在叶和根中同时诱导表达。有趣的是，两种亚型在感染后在所有三种组织（叶、根和茎）中表达，而对于另外两种成员没有检测到诱导。儿茶素和表儿茶素在根和茎中积累，而咖啡酸和柠檬酸仅在受感染的根中观察到。在发病初期诱导叶片和茎产生脱落酸、水杨酸、茉莉酸（JA）、赤霉素和吲哚乙酸（IAA）。IAA和JA是唯一的激素被确定在感染的根在后期stages.ConclusionsThe诱导的几个PR-10基因的草莓植株感染V.大丽花后，建议PR-10基因的防御反应对这种病原体的作用。在感染的早期阶段产生的植物激素和在后期阶段产生的抗真菌代谢物假设它们与这种反应有关。研究结果可能会改善病原菌的控制措施。
BackgroundThe soil-borne vascular pathogen Verticillium dahliae causes severe wilt symptoms in a wide range of plants including strawberry (Fragaria x ananassa). To enhance our understanding of the effects of V. dahliae on the growth and development of F. x ananassa, the expression patterns of 21 PR-10 genes were investigated by qPCR analysis and metabolite changes were determined by LC-MS in in vitro F. x ananassa plants upon pathogen infection.ResultsThe expression patterns of the 21 isoforms showed a wide range of responses. Four PR-10 genes were highly induced in leaves upon pathogen infection while eight members were significantly up-regulated in roots. A simultaneously induced expression in leaves and roots was detected for five PR-10 genes. Interestingly, two isoforms were expressed upon infection in all three tissues (leaves, roots and stems) while no induction was detected for two other members. Accumulation of antifungal catechin and epicatechin was detected upon pathogen infection in roots and stems at late stages, while caffeic acid and citric acid were observed only in infected roots. Production of abscisic acid, salicylic acid, jasmonic acid (JA), gibberellic acid and indole acetic acid (IAA) was induced in infected leaves and stems at early stages. IAA and JA were the sole hormones to be ascertained in infected roots at late stages.ConclusionsThe induction of several PR-10 genes upon infection of strawberry plants with V. dahliae suggest a role of PR-10 genes in the defense response against this pathogen. Production of phytohormones in the early stages of infection and antifungal metabolites in late stages suppose that they are implicated in this response. The results may possibly improve the control measures of the pathogen.