Hairy Leaf 6, an AP2/ERF Transcription Factor, Interacts with OsWOX3B and Regulates Trichome Formation in Rice
Hairy Leaf 6, an AP2/ERF Transcription Factor, Interacts with OsWOX3B and Regulates Trichome Formation in Rice
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Hairy Leaf 6 是一种 AP2/ERF 转录因子，与 OsWOX3B 相互作用并调节水稻毛状体的形成
DOI：
10.1016/j.molp.2017.09.015
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发表时间：
2017
期刊：
影响因子：
27.5
通讯作者：
Yu Sibin
中科院分区：
文献类型：
--
作者：
Sun Wenqiang;Gao Dawei;Xiong Yin;Tang Xinxin;Xiao Xiongfeng;Wang Chongrong;Yu Sibin
毛状体的形成作为植物表皮细胞分化和细胞形态建成的一个机制模型已被广泛研究。然而，毛状体形成的遗传和分子机制（即，起始和延伸）在水稻中的作用仍然很大程度上不清楚。在这里，我们报告了一个AP 2/ERF转录因子，毛叶6（HL 6），它控制水稻毛状体的形成。功能分析表明，HL 6在水稻中转录调控毛状体的伸长，这依赖于功能性OsWOX 3B，OsWOX 3B是一个含有同源结构域的蛋白，在毛状体起始中起关键调节作用。生物化学和分子遗传学分析表明，HL 6与OsWOX 3B之间存在物理相互作用，二者共同调控着毛状体形成过程中生长素相关基因的表达，其中OsWOX 3B可能增强HL 6与其直接靶基因OsYUCCA 5的结合能力。群体遗传分析表明，HL 6号在水稻驯化过程中处于负选择状态。总之，我们的研究结果提供了新的见解，在水稻毛状体形成的分子调控网络。
Trichome formation has been extensively studied as a mechanistic model for epidermal cell differentiation and cell morphogenesis in plants. However, the genetic and molecular mechanisms underlying trichome formation (i.e., initiation and elongation) in rice remain largely unclear. Here, we report an AP2/ERF transcription factor, Hairy Leaf 6 (HL6), which controls trichome formation in rice. Functional analyses revealed thatHL6transcriptionally regulates trichome elongation in rice, which is dependent on functional OsWOX3B, a homeodomain-containing protein that acts as a key regulator in trichome initiation. Biochemical and molecular genetic analyses demonstrated that HL6 physically interacts with OsWOX3B, and both of them regulate the expression of some auxin-related genes during trichome formation, in which OsWOX3B likely enhances the binding ability of HL6 with one of its direct target gene,OsYUCCA5. Population genetic analysis indicated thatHL6was under negative selection during rice domestication. Taken together, our findings provide new insights into the molecular regulatory network of trichome formation in rice.