Vaccination with nontoxic mutant toxic shock syndrome toxin 1 protects against Staphylococcus aureus infection
Vaccination with nontoxic mutant toxic shock syndrome toxin 1 protects against Staphylococcus aureus infection
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DOI：
10.1086/377308
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发表时间：
2003-09-01
影响因子：
6.4
通讯作者：
Nakane, A
中科院分区：
文献类型：
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作者：
Hu, DL;Omoe, K;Nakane, A
为了研究无毒突变型中毒性休克综合征毒素1（mTSST-1）疫苗接种小鼠是否能保护小鼠免受金黄色葡萄球菌感染，用mTSST-1疫苗接种小鼠，并用活的金黄色葡萄球菌攻击小鼠。金黄色。mTSST-1接种组的存活率更高，器官中的细菌计数显著低于对照小鼠。mTSST-1特异性抗体的被动转移也提供了针对S.金黄色葡萄球菌引起的脓毒性死亡与对照组相比，免疫小鼠血清样品和脾脏中干扰素（IFN）-γ的产生显著降低，而免疫小鼠中白细胞介素-10滴度显著升高。IFN-γ和肿瘤坏死因子-α在体外的生产显着抑制mTSST-1免疫小鼠的血清样品，但不从对照小鼠。这些结果表明，用缺乏超抗原特性的mTSST-1接种疫苗可提供针对沙门氏菌的保护。金黄色葡萄球菌感染，保护可能是介导的TSST-1中和抗体以及下调IFN-γ的生产。
To investigate whether vaccination with nontoxic mutant toxic shock syndrome toxin 1 (mTSST-1) can protect against Staphylococcus aureus infection, mice were vaccinated with mTSST-1 and challenged with viable S. aureus. Survival in the mTSST-1-vaccinated group was higher, and bacterial counts in organs were significantly lower than those of control mice. Passive transfer of mTSST-1-specific antibodies also provided protection against S. aureus-induced septic death. Interferon (IFN)-gamma production in the serum samples and spleens from vaccinated mice was significantly decreased compared with that in controls, whereas interleukin-10 titers were significantly higher in vaccinated mice. IFN-gamma and tumor necrosis factor-alpha production in vitro were significantly inhibited by serum samples from mTSST-1-immunized mice but not from control mice. These results suggest that vaccination with mTSST-1 devoid of superantigenic properties provides protection against S. aureus infection and that the protection might be mediated by TSST-1-neutralizing antibodies as well as by the down-regulation of IFN-gamma production.