Genetic Analysis of DEFECTIVE KERNEL1 Loop Function in Three-Dimensional Body Patterning in Physcomitrella patens1[C][W][OPEN]
Genetic Analysis of DEFECTIVE KERNEL1 Loop Function in Three-Dimensional Body Patterning in Physcomitrella patens1[C][W][OPEN]
复制标题
立碗藓 1 三维体图案中缺陷 KERNEL1 循环功能的遗传分析[C][W][OPEN]
作者：
V. Demko;P. Perroud;Wenche Johansen;C. Delwiche;E. Cooper;Pål Remme;A. Ako;Karl G. Kugler;K. Mayer;R. Quatrano;O. Olsen
提出的DEK1跨膜结构域的调控环片段是形成小球藻配子体模式所必需的。高等植物缺陷蛋白KERNEL1(DEK1)在位置依赖性信号转导中起着重要作用，它由一个大的跨膜结构域(MEM)连接到一个蛋白酶催化结构域和一个调控结构域组成。在这里，我们证明了假设的MEM结构域的感觉环在小立苔藓DEK1活性的发育调节中发挥着重要作用。与缺乏DEK1(∆DEK1)的P.patens相比，DEK1∆环突变体正确地将分裂平面定位在芽尖细胞中。与∆dek1芽的早期发育停滞相反，dek1∆环由于有丝分裂活动的错误调节而产生缺乏扩展叶柄的异常配子柄。与蛋白酶催化结构域的高度保守序列相比，Loop在陆地植物中是高度可变的。在功能上，地钱草的序列与DEK1的∆环表型完全互补，而玉米(玉米)和拟南芥(拟南芥)的序列给出的表型生长迟缓，叶状体发育受到影响。生物信息学分析确定MEM是主要促进剂超家族的成员，膜转运蛋白对来自外部环境的刺激做出反应。转录组分析比较野生型和∆dek1组织发现了对两组与dek1突变表型相关的转录本的影响：与细胞壁重塑和AINTEGUMENTA、PULTHORA和Baby BOOM2(APB2)和APB3转录因子的调节有关的转录本。最后，序列数据支持这样一种假设，即进化成祖先陆地植物的高级轮藻失去了胞质钙调蛋白，保留了DEK1作为进化中陆地植物谱系中唯一的钙调蛋白。
A proposed regulatory loop segment of the DEK1 transmembrane domain is required for gametophore patterning in Physcomitrella patens. DEFECTIVE KERNEL1 (DEK1) of higher plants plays an essential role in position-dependent signaling and consists of a large transmembrane domain (MEM) linked to a protease catalytic domain and a regulatory domain. Here, we show that the postulated sensory Loop of the MEM domain plays an important role in the developmental regulation of DEK1 activity in the moss Physcomitrella patens. Compared with P. patens lacking DEK1 (∆dek1), the dek1∆loop mutant correctly positions the division plane in the bud apical cell. In contrast with an early developmental arrest of ∆dek1 buds, dek1∆loop develops aberrant gametophores lacking expanded phyllids resulting from misregulation of mitotic activity. In contrast with the highly conserved sequence of the protease catalytic domain, the Loop is highly variable in land plants. Functionally, the sequence from Marchantia polymorpha fully complements the dek1∆loop phenotype, whereas sequences from maize (Zea mays) and Arabidopsis (Arabidopsis thaliana) give phenotypes with retarded growth and affected phyllid development. Bioinformatic analysis identifies MEM as a member of the Major Facilitator Superfamily, membrane transporters reacting to stimuli from the external environment. Transcriptome analysis comparing wild-type and ∆dek1 tissues identifies an effect on two groups of transcripts connected to dek1 mutant phenotypes: transcripts related to cell wall remodeling and regulation of the AINTEGUMENTA, PLETHORA, and BABY BOOM2 (APB2) and APB3 transcription factors known to regulate bud initiation. Finally, sequence data support the hypothesis that the advanced charophyte algae that evolved into ancestral land plants lost cytosolic calpains, retaining DEK1 as the sole calpain in the evolving land plant lineage.
影响因子：
56.9
作者：
Rensing, Stefan A.;Lang, Daniel;Boore, Jeffrey L.
通讯作者：
Boore, Jeffrey L.
影响因子：
3.1
作者：
Zimmer, Andreas;Lang, Daniel;Rensing, Stefan A.
通讯作者：
Rensing, Stefan A.
DOI：
10.1073/pnas.1218836109
发表时间：
2012-12-26
影响因子：
11.1
作者：
Ruessmann, Florian;Stemp, Markus J.;Hartl, F. Ulrich
通讯作者：
Hartl, F. Ulrich