Prenatal exposure to nicotine impairs protective responses of rat pups to hypoxia in an age-dependent manner
Prenatal exposure to nicotine impairs protective responses of rat pups to hypoxia in an age-dependent manner
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DOI：
10.1016/s0034-5687(01)00232-8
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发表时间：
2001-08-01
期刊：
影响因子：
--
通讯作者：
Ng, VKY
中科院分区：
文献类型：
--
作者：
Fewell, JE;Smith, FG;Ng, VKY
对幼鼠进行了实验，以研究产前接触尼古丁和出生后年龄之间的相互作用，以促进缺氧期间促进生存的保护反应。从妊娠第 6 天或第 7 天开始，怀孕大鼠通过 28 天的渗透微型泵连续接受尼古丁（类似于每天每公斤体重 6 毫克酒石酸尼古丁）或载体。在出生后第 1-2、5-6 和 10-11 天，幼崽要么暴露在通过呼吸缺氧气体混合物（97% N-2 和 3% CO2）产生的单次缺氧中，并确定其最后一次喘气的时间，要么反复暴露在缺氧中，并确定其从原发性呼吸暂停中自动复苏的能力。产前接触尼古丁会缩短最后喘息的时间。但仅限于 1-2 日龄的动物。然而，喘息的总数是……。由于尼古丁对喘息模式的影响，该年龄组的呼吸频率有所增加。此外。产前接触尼古丁会减少 1-2 日龄和 5-6 日龄幼犬的成功自动复苏次数，并影响死亡前的心肺事件，但不会影响 10-11 日龄幼犬。因此，我们的实验表明，产前接触尼古丁会损害幼鼠的保护反应，这些保护反应可能以年龄依赖性方式在暴露于缺氧期间维持生命。 (C) 2001 年爱思唯尔科学 BN。版权所有。
Experiments were carried out on rat pups to investigate the interaction between prenatal exposure to nicotine and postnatal age on protective responses that promote survival during exposure to hypoxia. From days 6 or 7 of gestation, pregnant rats received either nicotine ( similar to 6 mg of nicotine tartrate/kg of body weight per day) or vehicle continuously via a 28-day osmotic minipump. On postnatal days 1-2, 5-6 and 10-11, the pups were exposed either to a single period of hypoxia produced by breathing an anoxic gas mixture (97% N-2 and 3% CO2) and their time to last gasp determined., or they were exposed repeatedly to hypoxia and their ability to autoresuscitate from primary apnea determined. Prenatal exposure to nicotine decreased the time to last gasp. but only in the 1-2-day-old animals. The total number of gasps was., however. increased in this age group due to the effect of nicotine on the gasping pattern. Furthermore. prenatal exposure to nicotine decreased the number of successful autoresuscitations and influenced the cardiorespiratory events preceding death in the 1-2- and 5-6-day-old pups but not in the 10-11-day-old pups. Thus, our experiments show that prenatal exposure to nicotine impairs protective responses of rat pups that may sustain life during exposure to hypoxia in an age-dependent manner. (C) 2001 Elsevier Science BN. All rights reserved.