Linkage in cultured Chinese hamster cells of two genes, emtB and leuS, involved in protein synthesis and isolation of cell lines with mutations in three linked genes
Linkage in cultured Chinese hamster cells of two genes, emtB and leuS, involved in protein synthesis and isolation of cell lines with mutations in three linked genes
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培养的中国仓鼠细胞中参与蛋白质合成的两个基因 emtB 和 leuS 的连锁以及具有三个连锁基因突变的细胞系的分离
作者：
J. Wasmuth;L. Chu
我们已经确定，通过分离分析，从适当的杂交种，两个基因参与蛋白质合成，一个编码的核糖体蛋白（emtB）和一个编码亮氨酰-tRNA合成酶（leuS），共分离在一个非常高的频率，并在中国仓鼠卵巢和肺细胞。与此相反，emtA基因座，定义的第二互补组的依米丁抗性突变体，也有改变影响蛋白质合成，可能是核糖体，不与leuS。此外，我们已经确定，第三个基因，一个可以改变，以产生耐铬酸盐，是同线与emtB和leuS。我们已经选择了在这三个连锁基因的每一个突变的细胞系，并已表明，这三个基因座共分离的频率很高。由于这三个连锁基因的突变提供了容易区分的表型，这些细胞系应提供一个强大的工具，检查几个重要的问题，有丝分裂重组体细胞。
We have determined via segregation analyses from appropriate hybrids that two genes involved in protein synthesis, one encoding for a ribosomal protein (emtB) and one encoding for leucyl-tRNA synthetase (leuS), cosegregate at a very high frequency and are linked in both Chinese hamster ovary and lung cells. In contrast, the emtA locus, defined by a second complementation group of emetine-resistant mutants which also have alterations affecting protein synthesis and probably the ribosome, is not linked to leuS. In addition, we have determined that a third gene, one that can be altered to give rise to chromate resistance, is syntenic with emtB and leuS. We have selected cell lines with mutations in each of these three linked genes and have shown that the three loci cosegregate at a high frequency. Because the mutations in these three linked genes provide easily distinguishable phenotypes, these cell lines should provide a powerful tool for examining several important questions concerning mitotic recombination in somatic cells.