A 2-Mb YAC contig and physical map of the natural killer gene complex on mouse chromosome 6
A 2-Mb YAC contig and physical map of the natural killer gene complex on mouse chromosome 6
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DOI：
10.1006/geno.1997.4721
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发表时间：
1997-05-15
期刊：
影响因子：
4.4
通讯作者：
Yokoyama, WM
中科院分区：
文献类型：
--
作者：
Brown, MG;Fulmek, S;Yokoyama, WM
我们构建了小鼠 6 号染色体上 >2 Mb 区域的物理图谱，其中包含自然杀伤基因复合体 (NKC)。该图谱包含 14 个重叠的酵母人工染色体的重叠群，我们在其上放置了 25 个 NKC 标记。 NKC 遗传连锁基因编码超过 17 种蛋白质，这些蛋白质直接控制先天 NR 细胞介导的肿瘤溶解和抗病性。在此，我们表明 Nkrp1 基因聚集在 A2m 和 Cd69 基因两侧的区域，并且大多数 Ly49 基因聚集在类似于 1 Mb 距离的远端区域。重要的是，包括 NKC 在内的小鼠 6 号染色体和人类 12p 号染色体的同线性区间是保守的。 NKC 物种保护表明，人类 NKC 可能含有小鼠病毒抗病基因 Cmv1 和 Rmp1 的直系同源物。高分辨率 NKC 图谱将有助于研究 NKC 基因调控和鉴定表型定义的基因产物，这些产物赋予 NR 细胞针对病毒病原体的防御能力。 (C) 1997 年学术出版社。
We have constructed a physical map of a >2-Mb region on mouse chromosome 6 that contains the natural killer gene complex (NKC). The map comprises a contig of 14 overlapping yeast artificial chromosomes onto which we positioned 25 NKC markers. NKC genetically linked genes encode >17 proteins that directly control innate NR cell-mediated tumor lysis and disease resistance. Herein we show that Nkrp1 genes are clustered in a region flanked by A2m and Cd69 genes and that most Ly49 genes are clustered in a distal region similar to 1 Mb distant. Importantly, syntenic intervals of mouse chromosome 6 and human chromosome 12p that include the NKC are conserved. NKC species conservation suggests that the human NKC may contain orthologues for the mouse viral disease resistance genes, Cmv1 and Rmp1. The high-resolution NKC map will facilitate investigation of NKC gene regulation and identification of phenotypically defined gene products that confer NR cell defense against viral pathogens. (C) 1997 Academic Press.