Love on wings, a Dof family protein regulates floral vasculature in Vigna radiata
Love on wings, a Dof family protein regulates floral vasculature in Vigna radiata
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LOVE ON WINGS，一种 Dof 家族蛋白，调节绿豆花的脉管系统
DOI：
10.1186/s12870-019-2099-x
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发表时间：
2019-11-14
影响因子：
5.3
通讯作者：
Su, Shihao
中科院分区：
文献类型：
--
作者：
Guo, Wuxiu;Zhang, Xue;Su, Shihao
背景植物与传粉者之间的相互作用是丰富花部性状（传粉综合征）和促进开花植物多样化的重要因素。蜜蜂授粉的综合征之一，Faboideae龙骨花是形成一个着陆平台的翅膀和龙骨花瓣。然而，制作龙骨开花的分子机制仍不清楚。结果通过大规模诱变，从绿豆中分离到一个突变体Love on Wings（low），该突变体在花瓣不对称性和脉管系统发育方面存在缺陷，导致不能形成着陆平台。我们通过图位克隆和RNA测序（RNA-seq）分析克隆了该基因座。我们发现LOW基因编码一个核定位的Dof样蛋白，并在花的原维管和维管组织中表达。在豆科植物中检测到一个LOW拷贝，与其他类群相比，似乎至少有2个拷贝。目前已鉴定出31个Dof蛋白，并将其分为4个主要的直链组群（Major Cluster of Orthopathy Groups，MCOG）。我们还发现，在拟南芥中的异位表达的低驱动其天然启动子引起的花瓣脉管系统模式的变化。结论总之，我们的研究从V. radiata中分离出一种豆科植物Dof样因子LOW，它影响该物种的脉管系统发育，这种变化反过来又会影响龙骨开花的花瓣发育和整体形态。
Background The interaction among plants and their pollinators has been a major factor which enriched floral traits known as pollination syndromes and promoted the diversification of flowering plants. One of the bee-pollination syndromes in Faboideae with keel blossoms is the formation of a landing platform by wing and keel petals. However, the molecular mechanisms of elaborating a keel blossom remain unclear. Results By performing large scale mutagenesis, we isolated and characterized a mutant in Vigna radiata, love on wings (low), which shows developmental defects in petal asymmetry and vasculature, leading to a failure in landing platform formation. We cloned the locus through map-based cloning together with RNA-sequencing (RNA-seq) analysis. We found that LOW encoded a nucleus-localized Dof-like protein and was expressed in the flower provascular and vascular tissues. A single copy of LOW was detected in legumes, in contrast with other taxa where there seems to be at least 2 copies. Thirty one Dof proteins have been identified from the V. radiata's genome, which can be further divided into four Major Cluster of Orthologous Groups (MCOGs). We also showed that ectopic expression of LOW in Arabidopsis driven by its native promoter caused changes in petal vasculature pattern. Conclusions To summarize, our study isolated a legume Dof-like factor LOW from V. radiata, which affects vasculature development in this species and this change can, in turn, impact petal development and overall morphology of keel blossom.