APCs present Aβk-derived peptides that are autoantigenic to type B T cells
APCs present Aβk-derived peptides that are autoantigenic to type B T cells
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DOI：
10.4049/jimmunol.170.8.4155
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发表时间：
2003-04-15
影响因子：
4.4
通讯作者：
Unanue, ER
中科院分区：
文献类型：
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作者：
Lovitch, SB;Walters, JJ;Unanue, ER
B型T细胞识别外源性提供的多肽，但忽略来自细胞内处理的相同表位。在这项研究中，我们证明了B型T细胞的存在，从I-A(K)β链的加工中获得了丰富的自体多肽。针对该多肽的T细胞杂交瘤不能识别同基因的APC，尽管存在大量天然处理的表位，但对外源性多肽的反应呈剂量依赖关系。此外，这些杂交瘤对从表达I-A(K)的APC表面提取的Abeta(K)肽有反应。这种多肽是从B细胞系中分离出来的，在B细胞系中发现它的丰度很高；它也存在于缺乏人类白细胞抗原-DM的系中，但数量相当少。因此，B型T细胞存在于丰富的自体多肽的原始谱系中。我们讨论了这些发现对B型T细胞在免疫反应和自身免疫病理中的潜在生物学作用的影响。
Type B T cells recognize peptide provided exogenously but are ignorant of the same epitope derived from intracellular processing. In this study, we demonstrate the existence of type B T cells to an abundant autologous peptide derived from processing of the I-A(k) beta-chain. T cell hybridomas raised against this peptide fail to recognize syngeneic APC despite abundant presentation of the naturally processed epitope but react in a dose-dependent manner to exogenous peptide. Moreover, these hybridomas respond to Abeta(k) peptide extracted from the surface of I-A(k)-expressing APC. This peptide was isolated from B cell lines where it was found in high abundance; it was also present in lines lacking HLA-DM, but in considerably lower amounts. Therefore, type B T cells exist in the naive repertoire to abundant autologous peptides. We discuss the implications of these findings to the potential biological role of type B T cells in immune responses and autoimmune pathology.