Genomic characterization of a newly established esophageal squamous cell carcinoma cell line from China and published esophageal squamous cell carcinoma cell lines
Genomic characterization of a newly established esophageal squamous cell carcinoma cell line from China and published esophageal squamous cell carcinoma cell lines
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中国新建立的食管鳞状细胞癌细胞系和已发表的食管鳞状细胞癌细胞系的基因组特征
DOI：
10.1186/s12935-020-01268-x
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发表时间：
2020-05-24
影响因子：
5.8
通讯作者：
Su, Min
中科院分区：
文献类型：
--
作者：
Li, Xiang;Tian, Dongping;Su, Min
食管鳞状细胞癌（Esophageal squamous cell carcinoma，ESCC）是世界范围内最常见的恶性肿瘤之一，也是癌症相关死亡的主要原因之一。细胞系是基础医学研究中广泛使用的疾病模型，然而，在中国，具有良好特性的食管鳞癌细胞模型报道较少。方法CSEC 216来源于沿岸的潮汕地区1例45岁男性食管鳞癌患者。将标本切碎，接种于T-25烧瓶中进行原代培养。免疫荧光染色鉴定细胞来源及增殖活性。transwell法检测其体外迁移和侵袭能力。DNA短串联重复序列分析用于细胞授权。核型分析采用光谱核型分析法。利用全基因组测序来研究基因组改变。结果CSEC 216细胞系为未污染细胞系，细胞形态呈多角形，单层贴壁生长，具有上皮细胞特征。免疫组化显示其来源于上皮细胞，增殖速度快。群体倍增时间为29.7h。核型显示肿瘤细胞的模式与非整倍体和复杂的染色体畸变。结论中国食管癌高发区的食管癌细胞系CSEC 216与已发表的ESCC细胞系的突变谱、SNA或CNA基因相似，无交叉污染。生物学特性进行了研究。对CSEC 216和28 ESCC细胞系的基因组突变特征进行了表征，这提供了有助于未来使用的全面的细胞遗传学背景。
BackgroundEsophageal squamous cell carcinoma (ESCC) is one of the most prevalent malignancies and a major cause of cancer related death worldwide, especially in China. Cell lines are widely used disease models for basic medical research, however, well characterized ESCC cell models from China were seldom reported. Misidentifying and cross-contaminations of cell lines also hamper the way of producing solid and reproductive data.MethodsCSEC216 was originated from a 45-year-old male ESCC patient from Chaoshan littoral, China. Specimens were minced into fragments and seeded in T-25 flask for primary culture. Immunoflourescence staining was performed for identifying the origination and proliferation activity. In vitro migration and invasion abilities was tested by transwell assay. DNA Short Tandem Repeats profiling was implemented for cell authorization. Karyotype was investigated by spectrum karyotyping. Whole genome sequencing was utilized to investigate genomic alterations. Background information and genomic mutation data of published ESCC cell lines were obtained from online databases.ResultsCSEC216 was an uncontaminated cell line, exhibited epithelial cell features with polygonal morphology and adherent growth as monolayer. Immuno staining demonstrated its epithelial origination and high proliferation rate. The Population Doubling time was 29.7 h. The karyotype demonstrated tumor cell patterns with aneuploidy and complex chromosomal aberrations. Mutation signatures, genes with SNA or CNA of CSEC216 and published ESCC cell lines were similar with the mutation spectrum of original ESCC tumors.ConclusionsESCC cell line CSEC216 from high incidence region in China was established with no cross-contamination. Biological features were studied. Genomic mutation features of CSEC216 and 28 ESCC cell lines were characterized which provided thorough cytogenetic background that facilitated future usage.