spoT-dependent accumulation of guanosine tetraphosphate in response to fatty acid starvation in Escherichia coli.
spoT-dependent accumulation of guanosine tetraphosphate in response to fatty acid starvation in Escherichia coli.
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DOI：
10.1073/pnas.90.23.11004
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发表时间：
1993-12
影响因子：
11.1
通讯作者：
Manouchehr Seyfzadeh;John Keener;Masayasu Nomura
中科院分区：
文献类型：
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作者：
Manouchehr Seyfzadeh;John Keener;Masayasu Nomura
我们以前分离的大肠杆菌的突变体，优先影响rRNA的合成，并在基因（accD）编码乙酰辅酶A羧化酶的一个亚基突变。使用该突变体和脂肪酸和磷脂生物合成途径的其他突变体以及脂肪酸合成的特异性抑制剂浅蓝菌素，我们表明（i）在碳源和所有20种氨基酸存在下抑制脂肪酸合成刺激鸟苷四磷酸（ppGpp）的积累并导致优先抑制rRNA合成，（ii）这种ppGpp积累是spoT依赖性的，（iii）刺激这种spoT介导的反应的代谢信号的产生可能不依赖于磷脂饥饿或ATP水平的显著降低。
We previously isolated a mutant of Escherichia coli that is preferentially affected in the synthesis of rRNA and has a mutation in the gene (accD) encoding a subunit of acetyl-CoA carboxylase. Using this mutant and other mutants of the pathway for fatty acid and phospholipid biosynthesis as well as cerulenin, a specific inhibitor of fatty acid synthesis, we show that (i) inhibition of fatty acid synthesis in the presence of both a carbon source and all 20 amino acids stimulates the accumulation of guanosine tetraphosphate (ppGpp) and leads to preferential inhibition of rRNA synthesis, (ii) this ppGpp accumulation is spoT dependent, and (iii) the generation of the metabolic signal that stimulates this spoT-mediated response probably does not depend on either phospholipid starvation or a significant reduction in the level of ATP.