A genetic linkage map of Pleurotus pulmonarius based on AFLP markers, and localization of the gene region for the sporeless mutation
A genetic linkage map of Pleurotus pulmonarius based on AFLP markers, and localization of the gene region for the sporeless mutation
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DOI：
10.1139/g09-021
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发表时间：
2009-05-01
期刊：
影响因子：
3.1
通讯作者：
Matsumoto, Teruyuki
中科院分区：
文献类型：
--
作者：
Okuda, Yasuhito;Murakami, Shigeyuki;Matsumoto, Teruyuki
在食用菌的栽培中，包括肺侧耳（Pleenopulmonarius（Fr.）奎尔，子实体产生的大量孢子会对蘑菇种植者的健康、蘑菇栽培设施和自然种群的遗传多样性产生不利影响。在这项研究中，我们构建了一个主要的遗传连锁图谱，并确定了与孢子形成缺陷（无孢子）突变的肺原杆菌使用150个后代菌株来自无孢子和野生型菌株之间的杂交。根据300个AFLP标记的分离，两个交配型因素，和无孢子性状，一个连锁图，由12个连锁群。该图谱覆盖的遗传距离为971 cM，平均标记间距为5.2 cM。无孢子突变的基因区域位于连锁群II，包括40个AFLP标记和A交配型因子位点。在这些标记中，离无孢子位点最近的标记位于1.4cM远。构建肺原杆菌遗传连锁图谱并鉴定与无孢子位点紧密连锁的标记将有助于标记辅助选择育种具有重要经济性状的无孢子菌株。
In the cultivation of edible mushrooms, including Pleurotus pulmonarius (Fr.) Quel., the enormous number of spores produced by fruiting bodies can adversely affect mushroom growers' health, mushroom cultivation facilities, and the genetic diversity of natural populations. In this study, we constructed a primary genetic linkage map and identified the locus associated with the sporulation-deficient (sporeless) mutation of P. pulmonarius using 150 progeny isolates derived from a cross between sporeless and wild-type isolates. Based on the segregation of 300 AFLP markers, two mating-type factors, and the sporeless trait, a linkage map was generated consisting of 12 linkage groups. The map covered a total genetic distance of 971 cM, with an average marker interval of 5.2 cM. The gene region responsible for the sporeless mutation was located in linkage group II including 40 AFLP markers and the A mating-type factor locus. Of these markers, the nearest marker to the sporeless locus was located 1.4 cM away. Construction of this P. pulmonarius genetic linkage map and identification of markers that are closely linked to the sporeless locus will facilitate marker-assisted selective breeding of a sporeless strain with economically important traits.