Nanocomposites based on graphene oxide and mesoporous silica nanoparticles: Preparation, characterization and nanobiointeractions with red blood cells and human plasma proteins
Nanocomposites based on graphene oxide and mesoporous silica nanoparticles: Preparation, characterization and nanobiointeractions with red blood cells and human plasma proteins
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DOI：
10.1016/j.apsusc.2017.12.082
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发表时间：
2018-04-15
影响因子：
6.7
通讯作者：
Alves, Oswaldo L.
中科院分区：
文献类型：
--
作者：
Fonseca, Leandro C.;de Araujo, Maciel M.;Alves, Oswaldo L.
目前的工作是开发一种基于氧化石墨烯（GO）和介孔氨基二氧化硅纳米颗粒（H2N-MSNs）的新型纳米复合材料及其与红细胞（红细胞）和人体血浆的生物相互作用，以研究纳米生物相互作用。二氧化硅纳米颗粒和几种基于氧化石墨烯的材料分别以其高溶血潜能和与人类血浆蛋白的强相互作用而闻名。在此背景下，研究了GO-MSN相互作用及其对最小化报告效应的影响。该材料是通过酰胺化反应将H2N-MSNs共价附着在氧化石墨烯表面合成的。通过改变h2n - msn的质量，得到了GO-MSN纳米复合材料，并通过FTIR、NMR、XRD、TGA、zeta电位和TEM对其进行了表征。表征结果证实了纳米复合材料的制备，表明共价键的连接主要是通过胺-环氧反应进行的，并且证明了h2n - msn对氧化石墨烯的意外还原反应，并提出了其机理。生物测定显示溶血（红细胞溶解）减少，与人血浆蛋白（蛋白冠形成）的相互作用最小化。这些都是实现体内生物相容性和理解纳米生物相互作用的重要发现。最后，这项工作为GO-MSN纳米复合材料的新纳米医学应用开辟了可能性，例如药物递送系统。(C) 2017 Elsevier B.V.版权所有
The current work refers to the development of a novel nanocomposite based on graphene oxide (GO) and mesoporous amino silica nanoparticles (H2N-MSNs) and its biological interaction with red blood cells (RBCs) and human blood plasma toward the investigation of nanobiointeractions. Silica nanoparticles and several graphene oxide-based materials are, separately, known for their high hemolytic potential and strong interaction with human plasma proteins. In this context, the GO-MSN interaction and its influence in minimizing the reported effects were investigated. The materials were synthesized by covalently attaching H2N-MSNs onto the surface of GO through an amidation reaction. GO-MSN nanocomposites were obtained by varying the mass of H2N-MSNs and were characterized by FTIR, NMR, XRD, TGA, zeta potential and TEM. The characterization results confirm that nanocomposites were obtained, suggest covalent bond attachment mostly by amine-epoxy reactions and evidence an unexpected reduction reaction of GO by H2N-MSNs, whose mechanism is proposed. Biological assays showed a decrease of hemolysis (RBC lysis) and a minimization of the interaction with human plasma proteins (protein corona formation). These are important findings toward achieving in vivo biocompatibility and understanding the nanobiointeractions. Finally, this work opens possibilities for new nanomedicine applications of GO-MSN nanocomposites, such as drug delivery system. (C) 2017 Elsevier B.V. All rights reserved.