p53 expression in rheumatoid and psoriatic arthritis synovial tissue and association with joint damage
p53 expression in rheumatoid and psoriatic arthritis synovial tissue and association with joint damage
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DOI：
10.1136/ard.2004.024430
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发表时间：
2005-02-01
影响因子：
27.4
通讯作者：
Cañete, JD
中科院分区：
文献类型：
--
作者：
Salvador, G;Sanmarti, R;Cañete, JD
背景资料：目的：分析类风湿关节炎（RA）和银屑病关节炎（PsA）患者滑膜组织中p53蛋白的表达及其与关节损害的关系。45例患者（27例RA，18例PsA）通过关节镜获得滑膜活检标本。获得接受关节镜检查的手、脚和关节的X线片，以评估是否存在糜烂性疾病。使用CD 4、CD 8、CD 138、CD 20和CD 68单克隆抗体（mAb）分析滑膜细胞群。用DO 7单克隆抗体对34例患者（18例RA，16例PsA）的p53蛋白进行了免疫组化检测。在11例早期RA患者中，采用Larsen-Scott方法分析了p53蛋白与1年放射学损害进展之间的关系。结果：16/18例RA患者（89%）和9/16例PsA患者（56%）检测到p53蛋白，但其在RA中的表达显著高于PsA。在RA中，p53表达与糜烂性疾病显著相关，其评分在放射学进展的患者中更高。CD 68表达也与RA的糜烂和放射学进展相关。PsA.Conclusions：这些结果表明，p53 ST的过度表达和关节损伤的关联是RA的特点，而不是PsA，和p53 ST表达可能是一个预后标志物在RA关节损伤之间的任何p53或CD 68和糜烂性疾病。
Background: Overexpression and functional mutations of p53 have been found in the synovial tissue (ST) of patients with rheumatoid arthritis ( RA), but their clinical significance remains unclear.Objective: To analyse p53 expression in the ST of patients with RA and psoriatic arthritis (PsA) and its association with joint damage.Methods: Synovial biopsy specimens were obtained by arthroscopy in 45 patients ( 27 RA, 18 PsA). Radiographs of hands, feet, and the joint undergoing arthroscopy were obtained to evaluate the presence of erosive disease. Synovial cell populations were analysed using CD4, CD8, CD138, CD20, and CD68 monoclonal antibodies (mAbs). The p53 protein was determined by immunohistology using DO7 mAb in 34 patients (18 RA, 16 PsA). In 11 patients with early RA, the association between p53 and 1 year progression of radiographic damage was analysed using the Larsen-Scott method.Results: The p53 protein was detected in 16/18 (89%) patients with RA and in 9/16 (56%) patients with PsA, but its expression in RA was significantly higher than in PsA. In RA, p53 expression was significantly associated with erosive disease, and its scores were higher in patients with radiological progression. CD68 expression was also associated with erosions and radiological progression in RA. No association was found between either p53 or CD68 and erosive disease in PsA.Conclusions: These results suggest that p53 ST overexpression and association with joint damage is characteristic of RA rather than PsA, and that p53 ST expression might be a prognostic marker of joint damage in RA.