D1 dopamine receptor agonist (SKF-38393) induction of Fos immunoreactivity in progestin receptor-containing areas of female rat brain.
D1 dopamine receptor agonist (SKF-38393) induction of Fos immunoreactivity in progestin receptor-containing areas of female rat brain.
复制标题
D1 多巴胺受体激动剂 (SKF-38393) 诱导雌性大鼠大脑中含孕激素受体区域的 Fos 免疫反应性。
DOI：
10.1046/j.1365-2826.1997.00594.x
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发表时间：
1997
影响因子：
3.2
通讯作者：
Blaustein,JD
中科院分区：
文献类型：
--
作者：
Meredith,JM;Auger,AP;Blaustein,JD
Injection of dopamine or dopamine receptor subtype agonists facilitates the expression of lordosis in estrogen‐primed female rats. The D1receptor specific agonist, SKF‐38393, facilitates lordosis in estradiol‐primed female rats via a process that requires progestin receptors. Based on these data, neuronal response to the D1receptor agonist SKF‐38393 was assessed by expression of the immediate early gene protein, Fos. In the first experiment we examined the modulation of Fos expression by D1agonists in progestin receptor‐containing areas of estradiol‐primed female rat brain. In the second experiment we examined if there are progestin receptor‐containing cells that respond to stimulation of D1receptors with increased Fos expression. Ten to 14 days following ovariectomy and stereotaxic surgery, animals were injected with 5 μg estradiol benzoate. Forty eight h later they were injected intracerebroventricularly with 100 ng of SKF‐38393 or saline. One h following injection animals were perfused, and brain sections immunostained for Fos protein. Results from the first experiment suggest that SKF‐38393 increased the total number of Fos immunoreactive cells in the mid‐ventromedial hypothalamic nucleus/ventrolateral portion (VMHVL), the caudal VMHVL, the paraventricular hypothalamic nucleus and the caudate putamen. In the medial preoptic area, the rostral VMHVL and the arcuate hypothalamic nucleus, there was a significant increase in the number of darkly stained Fos‐immunoreactive cells following the SKF‐38393 treatment. In the second study, SKF‐38393 increased the number of progestin receptor‐containing cells which contained Fos immunoreactivity in the caudal VMHVL. The results suggest potential sites of action for the facilitation of sexual behavior by centrally administered D1agonists.
