Laminin A is required for follicle cell-oocyte signaling that leads to establishment of the anterior-posterior axis in Drosophila
Laminin A is required for follicle cell-oocyte signaling that leads to establishment of the anterior-posterior axis in Drosophila
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DOI：
10.1016/s0960-9822(00)00514-5
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发表时间：
2000-06-01
期刊：
影响因子：
9.2
通讯作者：
Ruohola-Baker, H
中科院分区：
文献类型：
--
作者：
Deng, WM;Ruohola-Baker, H
果蝇前后 (AP) 轴的建立需要卵子发生过程中卵母细胞和周围体细胞卵泡细胞之间的信号传导 [1,2]，首先，来自卵母细胞（Gurken (Grk)，一种转化生长因子-α (TGF α) 同源物）的信号被预定的终末卵泡细胞接收，其中表皮生长因子受体 (EGFR) 途径被激活并诱导后命运 [2-4]，随后，后卵泡细胞向卵母细胞发送未识别的信号，从而导致其细胞骨架极性重组，这种重组对于母体决定簇的正确定位是必需的，例如 oskar (osk) 和 bicoid (bcd) mRNA，它们决定卵母细胞和随后胚胎的 AP 极性 [2]，我们在此表明，当编码细胞外基质成分层粘连蛋白 A 的基因 IanA 在后卵泡细胞中发生突变时，在卵泡细胞中，AP决定簇的定位在下面的卵母细胞中被破坏，在IanA突变细胞中后卵泡细胞分化和卵泡细胞顶端-基底极性不受影响，这表明层粘连蛋白A是来自后卵泡细胞极化卵母细胞的正确信号所必需的。这是细胞外基质参与主要身体轴的建立的第一个证据。
The establishment of the anterior-posterior (AP) axis in Drosophila melanogaster requires signaling between the oocyte and surrounding somatic follicle cells during oogenesis [1,2], First, a signal from the oocyte (Gurken (Grk), a transforming growth factor-alpha (TGF alpha) homolog) is received by predetermined terminal follicle cells in which the epidermal growth factor receptor (EGFR) pathway is activated and a posterior fate is induced [2-4], Later, the posterior follicle cells send an unidentified signal back to the oocyte, which leads to the reorganization of its cytoskeletal polarity, This reorganization is required for proper localization of maternal determinants, such as oskar (osk) and bicoid (bcd) mRNAs, that determine the AP polarity of the oocyte and the subsequent embryo [2], We show here that when the gene IanA, which encodes the extracellular matrix component laminin A, is mutated in posterior follicle cells, localization of AP determinants is disrupted in the underlying oocyte, Posterior follicle-cell differentiation and follicle cell apical-basal polarity are unaffected in the IanA mutant cells, suggesting that laminin A is required for correct signaling from the posterior follicle cells that polarizes the oocyte, This is the first evidence that the extracellular matrix is involved in the establishment of a major body axis.