The proteome of mouse vestibular hair bundles over development.
The proteome of mouse vestibular hair bundles over development.
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DOI：
10.1038/sdata.2015.47
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发表时间：
2015
期刊：
影响因子：
9.8
通讯作者：
Barr-Gillespie PG
中科院分区：
文献类型：
--
作者：
Krey JF;Sherman NE;Jeffery ED;Choi D;Barr-Gillespie PG
脊椎动物毛束的发育是一个精确的精心安排的事件，最终产生一个紧密有序的安排肌动蛋白丰富的静纤毛和一个单一的轴丝动纤毛。为了了解束的蛋白质组成在发育过程中的变化，我们分离束从年轻（出生后P4-P6天）和成熟（P21-P25）小鼠椭圆囊使用的扭断方法，然后其特征在于其组成蛋白使用液相色谱串联质谱与数据依赖性采集。使用MaxQuant和无标记定量，我们测量了两个束和整个胞果中蛋白质的相对丰度;两个部分之间的蛋白质丰度的比较允许计算束中的富集。这些数据可通过ProteomeXchange（标识符PXD 002167）获得，将有助于检查哺乳动物前庭束中存在的蛋白质及其浓度如何在发育过程中变化。
Development of the vertebrate hair bundle is a precisely orchestrated event that culminates in production of a tightly ordered arrangement of actin-rich stereocilia and a single axonemal kinocilium. To understand how the protein composition of the bundle changes during development, we isolated bundles from young (postnatal days P4-P6) and mature (P21-P25) mouse utricles using the twist-off method, then characterized their constituent proteins using liquid-chromatography tandem mass spectrometry with data-dependent acquisition. Using MaxQuant and label-free quantitation, we measured relative abundances of proteins in both bundles and in the whole utricle; comparison of protein abundance between the two fractions allows calculation of enrichment in bundles. These data, which are available via ProteomeXchange with identifier PXD002167, will be useful for examining the proteins present in mammalian vestibular bundles and how their concentrations change over development.