Gamma interferon production by cytotoxic T lymphocytes is required for resolution of Chlamydia trachomatis infection
Gamma interferon production by cytotoxic T lymphocytes is required for resolution of Chlamydia trachomatis infection
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DOI：
10.1128/iai.66.11.5457-5461.1998
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发表时间：
1998-11-01
影响因子：
3.1
通讯作者：
Starnbach, MN
中科院分区：
文献类型：
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作者：
Lampe, MF;Wilson, CB;Starnbach, MN
在这项研究中，我们使用了干扰素基因被破坏的小鼠(干扰素-伽马(-/-)小鼠)来研究这种细胞因子在解决沙眼衣原体感染中的作用。我们发现，与干扰素-γ(+/+)对照组相比，干扰素-γ(-/-)小鼠清除沙眼衣原体感染的能力受到了损害。激活的CD8(+)细胞毒性T淋巴细胞(CTL)在细胞内感染时分泌干扰素-γ，我们之前已经证明，沙眼衣原体特异性CTL株过继转移到感染的小鼠中可以减少沙眼衣原体的感染。在本研究中，我们发现，当这些干扰素-γ(+/+)CTL株被转移到衣原体感染的干扰素-γ(-/-)小鼠体内时，所转移的CTL不能克服在干扰素-γ(-/-)小鼠中看到的免疫缺陷。我们还表明，可以从感染沙眼衣原体的干扰素-γ缺陷小鼠身上培养出衣原体特异性CTL；然而，将干扰素-γ(-/-)CTL过继转移到感染的干扰素-γ(+/+)小鼠并不能降低感染水平。这些结果表明，CTL产生的干扰素-γ不足以克服干扰素-γ(-/-)小鼠在解决衣原体感染方面的缺陷，但在过继将CTL转移到干扰素-γ(+/+)小鼠体内后，需要由CTL产生干扰素-γ才能发挥保护作用。
In this study, we used mice in which the gene for gamma interferon (IFN-gamma) has been disrupted (IFN-gamma(-/-) mice) to study the role of this cytokine in the resolution of Chlamydia trachomatis infection. We show that IFN-gamma(-/-) mice are impaired in the ability to clear infection with C. trachomatis compared to IFN-gamma(+/+) control mice. Activated CD8(+) cytotoxic T lymphocytes (CTL) secrete IFN-gamma in response to intracellular infection, and we have shown previously that a Chlamydia-specific CTL line can reduce C. trachomatis infection when adoptively transferred into infected mice. In the present study, we found that when these IFN-gamma(+/+) CTL lines are transferred into Chlamydia-infected IFN-gamma(-/-) mice, the transferred CTL cannot overcome the immune defect seen in the IFN-gamma(-/-) mice. We also show that Chlamydia-specific CTL can be cultured from IFN-gamma-deficient mice infected with C, trachomatis; however, the adoptive transfer of IFN-gamma(-/-) CTL into infected IFN-gamma(+/+) mice does not reduce the level of infection. These results suggest that IFN-gamma production by CTL is not sufficient to overcome the defect that IFN-gamma(-/-) mice have in the resolution of Chlamydia infection, yet IFN-gamma production by CTL is required for the protective effect seen upon adoptive transfer of CTL into IFN-gamma(+/+) mice.