The vestibular club endings in Ameiurus - Further evidence on the morphology of the synapse
The vestibular club endings in Ameiurus - Further evidence on the morphology of the synapse
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DOI：
10.1002/cne.900570303
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发表时间：
1933-06-01
影响因子：
2.5
通讯作者：
Hoerr, NL
中科院分区：
文献类型：
--
作者：
Bartelmez, GW;Hoerr, NL
在鲶鱼（Ameiurus）中，来自球囊的粗髓鞘根纤维终止于毛特纳细胞侧树突上的棒状末端。该系统已经有 10 毫米的髓鞘。幼虫。所描述的突触成分的大尺寸(棒状末端高达7.2μ)使其有利于使用不受金属浸渍固有的异议的方法进行细胞学研究。在成人中，通过灌注和媒染进行固定对于保留末端是必要的，末端可以通过适当的染色与其他细胞突起和细胞周末端网区分开来。神经元在2-3μ切片中是明显的；它们纵向布置在球杆和侧枝晶中，因此这两组通常彼此成一定角度。它们不相互吻合或穿过突触膜。线粒体不规则地散布在树突中，但在球杆末端，它们通常聚集在突触表面附近。棒状末端与树突及其分支的关系使得5μ截面通常很难解释。 Bielschowsky 技术被谴责为对突触研究不可靠，因为误差源是由以下因素引入的：(1) 所采用的固定，(2) 髓鞘的破坏，以及 (3) 表面沉积物、结壳和通过对技术进行轻微修改可能产生的类似覆盖物。
In the catfish (Ameiurus) coarse myelinated root fibers from the sacculus terminate in club-shaped endings on the lateral dendrite of Mauthner''s cell. The system is already myelinated in 10-mm. larva. The large size of the components of the synapse described (club endings up to 7.2[mu]) makes it favorable for cytologic study with methods which are free from the objections inherent in metallic impregnations. In the adult, fixation by perfusion and mordanting are necessary to preserve the endings which can be differentiated by appropriate staining from other cell processes and the pericellular terminal net. Neuronbrillae are distinct in 2-3[mu] sections; they are longitudinally disposed in the clubs and the lateral dendrite so that the 2 sets usually run at an angle to each other. They do not anastomose with one another or cross the synaptic membrane. Mitochondria are irregularly scattered through the dendrite, but in the club endings they are often aggregated near the synaptic surface. The relations of the club endings to the dendrite and its branches are such that 5[mu] sections are often very difficult to interpret. The Bielschowsky technique is condemned as unreliable for the study of the synapse, since sources of error are introduced (1) by the fixation employed, (2) by the destruction of the myelin sheaths, and, (3) by the surface deposits, incrustations, and similar overlays that can be produced by slight modifications of the technique.