Defensing role of novel piscidins from largemouth bass (Micropterus salmoides) with evidence of bactericidal activities and inducible expressional delineation
Defensing role of novel piscidins from largemouth bass (Micropterus salmoides) with evidence of bactericidal activities and inducible expressional delineation
复制标题
大口黑鲈（Micropterus salmoides）新型鱼素的防御作用，具有杀菌活性和诱导表达描绘的证据
DOI：
10.1016/j.micres.2021.126953
复制
发表时间：
2022-03-01
影响因子：
6.7
通讯作者：
Yu, Haining
中科院分区：
文献类型：
--
作者：
Duan, Yuxin;Ouyang, Jianhong;Yu, Haining
大口黑鲈是重要的淡水养殖经济鱼类，对其免疫系统的深入研究是科普严重传染病的迫切需要。Piscidin是一个重要的抗菌肽家族，几乎存在于所有的硬骨鱼中。然而，在大口黑鲈中没有鱼杀菌素的报道。在本研究中，三个新的piscidin（MSPiscidin-1，-2，和-3），首次确定和表征大口黑鲈。MSPiscidin-1、MSPiscidin-2和MSPiscidin-3的预测成熟肽（分别由24、27、25个氨基酸残基组成）均采用代表阳离子AMP的两亲性α-螺旋构象，阳离子AMP对于膜透化和抗菌活性是重要的。MSPiscidin-2和MSPiscidin-3在体外对水生病原菌确实表现出强、广谱和高效的抗菌活性，但MSPiscidin-1没有表现出直接的抗菌活性。MSPiscidin-2和MSPiscidin-3主要通过诱导细菌细胞膜透化作用来杀灭细菌，此外，它们还能与细菌基因组DNA相互作用，影响DNA的复制和转录。此外，MSPiscidin-2和-3还能有效抑制细菌生物膜的形成并消除预先形成的生物膜。在体内实验中，MSPiscidin-1-3基因在哈氏弧菌感染的组织中表现出可诱导的表达模式，这进一步表明了piscidin在大口黑鲈天然免疫中的关键作用。总之，本研究将补充对M.本研究为水产养殖中新型肽类抗菌剂的设计提供了候选药物。
Micropterus salmoides is an economical important species of freshwater-cultured fish, the in-depth knowledge of its immune system is in urgent development to cope with serious infectious diseases. Piscidin is an important antimicrobial peptide (AMP) family existing in almost all teleosts. However, no piscidin has been reported in largemouth bass. In this study, three novel piscidins (MSPiscidin-1,-2, and-3) were firstly identified and characterized from the largemouth bass. The predicted mature peptides of MSPiscidin-1,-2, and-3 (consists of 24, 27, 25 amino acid residues, respectively) all adopted an amphipathic alpha-helical conformation representative of cationic AMPs that are important for membrane permeabilization and antibacterial activity. MSPiscidin-2 and-3 indeed displayed strong, broad-spectrum, and highly efficient antimicrobial activities in vitro against aquatic pathogens, but MSPiscidin-1 didn't show direct antimicrobial activity. MSPiscidin-2 and-3 killed bacteria mainly by inducing membrane permeabilization, in addition, they also can interact with bacterial genomic DNA, which might influence the DNA replication and transcription. Besides, MSPiscidin-2 and-3 could effectively inhibit the formation of the bacterial biofilm and eliminate the preformed biofilms. In vivo, MSPiscidin-1-3 genes showed an inducible expression pattern in the tested tissues upon Vibrio harveyi infection, which further indicated the key roles of piscidins in innate immunity in largemouth bass. Overall, this study will supplement the understanding of M. salmoides innate immune system and provide candidates for the design of novel peptide antibacterial agents used in aquaculture.