Red algae-derived isofloridoside activates the sweet taste receptor T1R2/T1R3
Red algae-derived isofloridoside activates the sweet taste receptor T1R2/T1R3
复制标题
红藻衍生的异花苷激活甜味受体 T1R2/T1R3
DOI：
10.1016/j.fbio.2022.102186
复制
发表时间：
2022
期刊：
影响因子：
5.2
通讯作者：
S. Mitsutake
中科院分区：
文献类型：
--
作者：
M. Akishino;Y. Aoki;H. Baba;M. Asakawa;Y. Hama;S. Mitsutake
异花糖苷（IF）是一种天然存在的半乳糖基甘油化合物，具有清爽的甜味。它存在于红藻中，在那里它作为光合作用的中间体。然而，IF增强甜味的机制尚未确定。在这项研究中，我们调查IF和甜味受体T1 R2/T1 R3之间的相互作用。我们发现，IF导致细胞内Ca 2+浓度增加和T1 R2/T1 R3表达的HEK 293 T细胞中Erk磷酸化增加，表明IF与T1 R2/T1 R3相互作用。IF还激活小鼠小肠内分泌L细胞中内源性表达的T1 R2/T1 R3，并促进肠促胰岛素胰高血糖素样肽-1（GLP-1）的分泌。IF和T1 R2/T1 R3的计算机对接模拟预测，IF与T1 R2的细胞外捕蝇草结构域中的Tyr 103形成氢键。这一结果与许多其他甜味分子的结合模式一致。我们发现IF具有作为一种新的替代甜味剂的潜力。
Isofloridoside (IF) is a naturally occurring galactosylglycerol compound with a refreshing sweet taste. It is found in red algae, where it acts as a photosynthetic intermediate. However, the mechanism by which IF elicits sweetness has not been determined. In this study, we investigate the interaction between IF and the sweet taste receptor T1R2/T1R3. We show that IF causes an increase in intracellular Ca2+concentration and an increase in Erk phosphorylation in T1R2/T1R3-expressing HEK293T cells, indicating that IF interacts with T1R2/T1R3. IF also activates endogenously expressed T1R2/T1R3 in mouse small intestinal endocrine L-cells, and promotes the secretion of the incretin glucagon-like peptide-1 (GLP-1).In silicodocking simulations of IF and T1R2/T1R3 predict that IF forms hydrogen bonds with Tyr103 in the extracellular Venus flytrap domain of T1R2. This result is consistent with the binding mode of many other sweet-tasting molecules. We found that IF have the potential as a new alternative sweetener.