Protein kinase A dependent membrane protein phosphorylation and chloride conductance in endosomal vesicles from kidney cortex.
Protein kinase A dependent membrane protein phosphorylation and chloride conductance in endosomal vesicles from kidney cortex.
复制标题
肾皮质内体囊泡中蛋白激酶 A 依赖性膜蛋白磷酸化和氯电导。
DOI：
10.1021/bi00116a026
复制
发表时间：
1992
期刊：
影响因子：
2.9
通讯作者：
Verkman,AS
中科院分区：
文献类型：
--
作者：
Reenstra,WW;Sabolic,I;Bae,HR;Verkman,AS
MethodsVesicle Preparations. For anion transport measurements, endosomes were labeled with SPQ in vivo (Bae & Verkman, 1990). New Zealand White rabbits were infused intravenously with 200 mg of SPQ in 10 mL of phosphate-buffered saline (PBS) and killed 10 min later. The renal arteries were perfused with 50 mL of cold PBS, and the superficial renal cortex (0.5-1-mm depth) was dissected. Under these conditions it was shown that> 95% of labeled endosomes originated from the apical membrane of proximaltubule cells (Lencer et al., 1990a). Renal cortex was homogenized in isethionate buffer (100 mM potassium isethionate, 100 mM mannitol, 1 mM magnesium acetate, 5 mM potassium phosphate, pH 7.4) by 10 strokes of the Potter-Elvehjem homogenizer. Large debris and mitochondria were removed by a low-speed centrifugation at 1000# for 10 min. A microsomal pellet containing SPQ-labeled endosomes was obtained by centrifugation of the su-pernatant at 100000# for 30 min. The microsomal pellet was washed once with isethionate buffer. The pellet was homo-
登录
查看更多内容
质子泵在大鼠肝脏多泡体中产生电化学梯度。
DOI：
--
发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
VanDyke,RW
通讯作者：
VanDyke,RW
哺乳动物近端小管中的氯转运。
DOI：
10.1007/bf00584945
发表时间：
1986
期刊：
Pflugers Archiv : European journal of physiology
影响因子：
--
作者：
Schild,L;Giebisch,G;Karniski,L;Aronson,PS
通讯作者：
Aronson,PS
DOI：
10.1016/0005-2736(81)90243-1
发表时间：
1981-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
BIBER, J;STIEGER, B;MURER, H
通讯作者：
MURER, H
来自肾皮质的囊泡中 ATP 驱动的质子运输。
影响因子：
--
作者：
I. Sabolić;G. Burckhardt
通讯作者：
G. Burckhardt
DOI：
10.1073/pnas.86.2.544
发表时间：
1989-01-01
影响因子：
11.1
作者：
CAIN, CC;SIPE, DM;MURPHY, RF
通讯作者：
MURPHY, RF