The outer membrane porin OmpW of Acinetobacter baumannii is involved in iron uptake and colistin binding
The outer membrane porin OmpW of Acinetobacter baumannii is involved in iron uptake and colistin binding
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DOI：
10.1002/1873-3468.12050
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发表时间：
2016-01-01
期刊：
影响因子：
3.5
通讯作者：
De, Emmanuelle
中科院分区：
文献类型：
--
作者：
Catel-Ferreira, Manuella;Marti, Sara;De, Emmanuelle
本研究旨在研究外膜蛋白OmpW的功能，该蛋白可能与鲍曼不动杆菌对粘菌素和亚胺培南耐药有关。OmpW在人工脂双层中的重组表明，它形成小通道(在1MKCl中为23ps)，并与铁和粘菌素显著相互作用，但不与亚胺培南相互作用。在体内，Fe-55摄取分析比较了ompW突变株和野生型菌株的行为，证实了OmpW在鲍曼不动杆菌铁稳态中的作用。然而，OmpW表达的缺失并没有影响鲍曼不动杆菌对粘菌素或亚胺培南的敏感性。
This study was undertaken to characterize functions of the outer membrane protein OmpW, which potentially contributes to the development of colistin- and imipenem-resistance in Acinetobacter baumannii. Reconstitution of OmpW in artificial lipid bilayers showed that it forms small channels (23 pS in 1 m KCl) and markedly interacts with iron and colistin, but not with imipenem. In vivo,Fe-55 uptake assays comparing the behaviours of ompW mutant and wild-type strains confirmed a role for OmpW in A. baumannii iron homeostasis. However, the loss of OmpW expression did not have an impact on A. baumannii susceptibilities to colistin or imipenem.