LOS2, a genetic locus required for cold-responsive gene transcription encodes a bi-functional enolase
LOS2, a genetic locus required for cold-responsive gene transcription encodes a bi-functional enolase
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DOI：
10.1093/emboj/21.11.2692
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发表时间：
2002-06-03
期刊：
影响因子：
11.4
通讯作者：
Zhu, JK
中科院分区：
文献类型：
--
作者：
Lee, H;Guo, Y;Zhu, JK
拟南芥突变体los 2在一定条件下损害冷响应基因的转录、获得性抗冻性和植物抗冷性。通过定位克隆分离出LOS 2，并显示其编码糖酵解途径中的烯醇化酶。在动物细胞中，烯醇化酶也被认为是一种转录因子，通过与c-myc基因启动子结合来抑制c-myc的表达。与绿色荧光蛋白融合的LOS 2靶向细胞核以及细胞质。LOS 2/烯醇化酶蛋白可以与人c-myc基因启动子的顺式元件和拟南芥锌指转录抑制子STZ/ZAT 10的基因启动子结合。STZ/ZAT 10表达在野生型中被冷快速和瞬时诱导，并且这种诱导在los 2突变体中更强和更持续。此外，RD 29 A-LUC报告基因的表达被STZ/ZAT 10在拟南芥叶片中的瞬时表达测定中显著抑制。我们的研究结果表明，植物中的冷响应基因的转录是由一个双功能烯醇化酶控制的。
The Arabidopsis mutation, los2, impairs cold-responsive gene transcription, acquired freezing tolerance and plant resistance to chilling under certain conditions. LOS2 was isolated through positional cloning and shown to encode an enolase in the glycolytic pathway. In animal cells, enolase has also been known to function as a transcription factor that represses the expression of c-myc by binding to the c-myc gene promoter. LOS2 fused to green fluorescent protein is targeted to the nucleus as well as to the cytoplasm. LOS2/enolase protein can bind to the cis-element of the human c-myc gene promoter and to the gene promoter of STZ/ZAT10, a zinc finger transcriptional repressor from Arabidopsis. STZ/ZAT10 expression is induced rapidly and transiently by cold in the wild type, and this induction is stronger and more sustained in the los2 mutant. Furthermore, the expression of a RD29A-LUC reporter gene is repressed significantly by STZ/ZAT10 in transient expression assays in Arabidopsis leaves. Our results demonstrate that cold-responsive gene transcription in plants is controlled by a bi-functional enolase.