Inhibition of Rev-mediated HIV-1 expression by an RNA binding protein encoded by the interferon-inducible 9-27 gene.
Inhibition of Rev-mediated HIV-1 expression by an RNA binding protein encoded by the interferon-inducible 9-27 gene.
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由干扰素诱导型 9-27 基因编码的 RNA 结合蛋白抑制 Rev 介导的 HIV-1 表达。
DOI：
10.1126/science.7680491
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Pavlakis,GN
中科院分区：
文献类型：
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作者：
Constantoulakis,P;Campbell,M;Felber,BK;Nasioulas,G;Afonina,E;Pavlakis,GN
干扰素通过未知机制抑制人类免疫缺陷病毒1型（HIV-1）的表达。通过筛选HIV-1病毒Rev-responsive element （RRE）结合蛋白的人互补DNA文库，分离出干扰素-α (IFN-α)和干扰素-γ (IFN-γ)诱导基因。该基因的产物RBP9-27在体外被证明可以结合RNA，并在转染到人细胞后抑制HIV-1的表达。RBP9-27主要抑制rev依赖的病毒基因表达的转录后步骤。因此，RBP9-27是拮抗Rev功能的细胞因子。这些结果表明，干扰素诱导的抗病毒机制通过诱导RNA结合蛋白（如RBP9-27）起作用。阐明RBP9-27的功能可能有助于更好地了解干扰素在HIV-1感染过程中的作用机制。
Interferon inhibits expression of human immunodeficiency virus type-1 (HIV-1) through unknown mechanisms. A gene inducible by interferon-α (IFN-α) and interferon-γ (IFN-γ) was isolated by screening of a human complementary DNA library for proteins binding to the Rev-responsive element (RRE) of HIV-1. The product of this gene, RBP9-27, was shown to bind RNA in vitro and to inhibit HIV-1 expression after transfection into human cells. RBP9-27 primarily inhibited Rev-dependent posttranscriptional steps of viral gene expression. Thus, RBP9-27 is a cellular factor that antagonizes Rev function. These results suggest an interferon-induced antiviral mechanism operating through the induction of RNA binding proteins such as RBP9-27. Elucidation of RBP9-27 function may lead to a better understanding of the mechanism of interferon action during HIV-1 infection.