Kaolin-reinforced 3D MBG scaffolds with hierarchical architecture and robust mechanical strength for bone tissue engineering.
Kaolin-reinforced 3D MBG scaffolds with hierarchical architecture and robust mechanical strength for bone tissue engineering.
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DOI：
10.1039/c4tb00025k
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发表时间：
2014-05
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影响因子：
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通讯作者：
Wei Tang;Yuan Yuan-Yuan;Dan Lin;Haoyi Niu;Changsheng Liu
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文献类型：
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作者：
Wei Tang;Yuan Yuan-Yuan;Dan Lin;Haoyi Niu;Changsheng Liu
三维介孔生物玻璃（3D MBG）支架具有介孔结构和高度互联的大孔网络，被认为是骨组织应用的理想生物材料。但其固有的脆性和较差的机械强度极大地阻碍了其性能和临床应用。本文以高岭土为粘结剂，采用改性聚氨酯泡沫塑料（PU）模板法，提出了一种制备具有优异机械强度、矿化能力和良好细胞反应的三维MBG支架的简便新方法。制备的混合MBG- xk（其中X为支架中高岭土的最终干重）支架的孔隙率为85%，随着高岭土含量的增加（5-20%），其抗压强度达到2.6 ~ 6.0 MPa，比传统pu模板MBG支架的抗压强度提高约100倍。添加高岭土后，MBG-10K支架具有更稳定和理想的pH环境，并增强了蛋白质吸附能力。此外，以大鼠骨髓基质细胞为模型，体外细胞培养实验表明，与MBG相比，制备的MBG- xk支架具有相当的细胞增殖，渗透能力，细胞附着和成骨分化能力，特别是MBG- 10k。
Three-dimensional mesoporous bioglass (3D MBG) scaffolds with mesoporous structures and highly interconnected macroporous networks are considered as ideal biomaterials for skeletal tissue applications. However, their inherent brittleness and poor mechanical strength greatly hamper their performance and clinical application. Here, using a modified polyurethane foam (PU) templating method with utilization of kaolin as binder, a new facile method for preparation of 3D MBG scaffolds with excellent mechanical strength, mineralization ability and desirable cellular response is proposed. The developed hybrid MBG-XK (where X refers to the final dry weight of kaolin in the scaffold) scaffolds with 85% porosity exhibited a high compressive strength from 2.6 to 6.0 MPa with increasing content of kaolin (5-20%), about 100 times higher than that of the traditional PU-template MBG scaffold. With the addition of kaolin, the MBG-10K scaffold exhibited a more stable and desirable pH environment, and an enhanced protein adsorption capacity. Furthermore, with rat bone marrow stromal cells as a model, in vitro cell culture experiments indicated that, compared with MBG, the prepared MBG-XK scaffolds possessed comparable cell proliferation, penetration capacity, enhanced cell attachment and osteogenic differentiation, especially for MBG-10K.