AN ADHESION-ASSOCIATED AGONIST FROM THE ZONA PELLUCIDA ACTIVATES G PROTEIN-PROMOTED ELEVATIONS OF INTERNAL CA-2+ AND PH THAT MEDIATE MAMMALIAN SPERM ACROSOMAL EXOCYTOSIS
AN ADHESION-ASSOCIATED AGONIST FROM THE ZONA PELLUCIDA ACTIVATES G PROTEIN-PROMOTED ELEVATIONS OF INTERNAL CA-2+ AND PH THAT MEDIATE MAMMALIAN SPERM ACROSOMAL EXOCYTOSIS
复制标题
DOI：
10.1016/0012-1606(89)90164-4
复制
发表时间：
1989-09-01
影响因子：
2.7
通讯作者：
BABCOCK, DF
中科院分区：
文献类型：
--
作者：
FLORMAN, HM;TOMBES, RM;BABCOCK, DF
溶解卵母细胞透明带促进顶体胞吐在呋喃-2-或羧基荧光素加载，成熟的牛精子。精子悬浮液中的内部[Ca 2 +]和pH值的相关升高在2-5分钟时首次检测到，没有明显的时间分辨率，此后单调增加。Fura-2负载的单细胞的视频成像鉴定了一个响应性亚群，注定要经历胞吐作用，其未显示早期短暂性，但表现出1-7分钟的滞后，然后持续升高内部[Ca 2 +]。两个透明带诱导的胞吐和染料反应减少功能不成熟的精子和成熟的精子与百日咳毒素初步处理。总之，这些结果表明，发育调节的信号转导机制采用G蛋白偶联生理性激动剂与顶体胞吐作用的内部离子介质的改变。
Solubilized oocyte zonae pellucidae promoted acrosomal exocytosis in fura-2- or carboxyfluorescein-loaded, mature bovine sperm. Associated elevations of internal [Ca2+] and pH in sperm suspensions were first detectable at 2-5 min, without apparent temporal resolution, and increased monotonically thereafter. Video imaging of fura-2-loaded, single cells identified a responsive subpopulation, destined to undergo exocytosis, that displayed no early transient but manifested lags of 1-7 min then sustained elevations of internal [Ca2+]. Both the zona-induced exocytosis and dye responses were diminished for functionally immature sperm and for mature sperm treated preliminarily with pertussis toxin. Together, these results indicate that a developmentally regulated mechanism of signal transduction employs G protein(s) to couple the physiological (zona) agonist to alterations of the internal ionic mediators of acrosomal exocytosis.