Effects of dietary phytol on tissue accumulation of phytanic acid and pristanic acid and on the tissue lipid profiles in mice
Effects of dietary phytol on tissue accumulation of phytanic acid and pristanic acid and on the tissue lipid profiles in mice
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DOI：
10.1111/asj.13424
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发表时间：
2020-01-01
影响因子：
2
通讯作者：
Kawahara, Satoshi
中科院分区：
文献类型：
--
作者：
Nakanishi, Tomonori;Kagamizono, Kazuhiro;Kawahara, Satoshi
最近的体外证据表明，植物醇源性脂肪酸植酸(PA)和伯斯坦酸(PRA)是动物产品中的成分，对人类健康既有有益的影响，也有有害的影响。在这项研究中，我们研究了PA和PRA在体内的组织积累和组织脂质谱的变化，使用含有植物酚的食物喂养的小鼠。治疗4周后，取血浆、脂肪组织、肝脏和脑组织，进行生化和气相色谱测定。饮食中的植醇可导致PA和PRA在脂肪组织和肝脏中蓄积，但不在大脑中蓄积，并降低血浆和肝脏三酰甘油水平。植物醇的摄入也降低了脂肪组织中的脂肪酸浓度，特别是亚油酸等多不饱和脂肪酸，但增加了这些脂肪酸在肝脏中的浓度。然而，饮食中的植醇对大脑脂质谱的影响很小。这项研究表明，摄入植物酚可导致脂肪组织和肝脏中PA和PRA的积累，并改变脂肪组织和肝脏的脂类分布，但大脑是一个不受饲料植物酚诱导的变化影响的组织。
Recent in vitro evidence suggests that the phytol-derived fatty acids, phytanic acid (PA) and pristanic acid (PrA), are components of animal products with the potential to cause both beneficial and harmful effects on human health. In this study, we investigated the in vivo tissue accumulation of PA and PrA and the changes in tissue lipid profiles, using mice fed a phytol-containing diet. After 4 weeks of treatment with a diet containing 1.0% phytol, plasma, adipose tissue, liver, and brain were collected and their lipid profiles were biochemically and gas-chromatographically determined. Dietary phytol caused PA and PrA accumulation in the adipose tissue and liver but not in the brain, and reduced plasma and liver triacylglycerol levels. Phytol intake also decreased the fatty acid concentrations in the adipose tissue, especially polyunsaturated fatty acids such as linoleic acid, but increased the concentrations of these fatty acids in the liver. However, dietary phytol had a low impact on the brain lipid profile. This study suggests that dietary phytol intake caused accumulation of PA and PrA and modified lipid profiles in the adipose tissue and liver, but that the brain is an insusceptible tissue to dietary phytol-induced changes.