Allergen concentration in natural rubber latex.
Allergen concentration in natural rubber latex.
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天然胶乳中的过敏原浓度。
DOI：
10.1111/j.1365-2222.2006.02531.x
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Biagini,RE
中科院分区：
文献类型：
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作者：
Yeang,H-Y;Hamilton,RG;Bernstein,DI;Arif,SAM;Chow,K-S;Loke,Y-H;Raulf-Heimsoth,M;Wagner,S;Breiteneder,H;Biagini,RE
背景巴西橡胶树乳胶血清含有完整的乳胶过敏原，常被用作乳胶过敏诊断的体内和体外参考抗原。目的本研究定量乳胶血清中重要过敏原的浓度，并检验其作为乳胶过敏诊断和免疫治疗中抗原源的适用性。方法从反复冻融或甘油化的离心乳胶中提取血清相。通过双位点免疫酶测定对乳胶过敏原进行定量。乳胶过敏原的 RNA 转录物丰度是根据表达序列标签库中克隆的数量来估计的。结果在冻融和甘油化乳胶血清中检测到乳胶过敏原 Hev b 1、2、3、4、5、6、7 和 13，其水平范围为 75 (Hev b 6) 至 0.06nmol/mg 总蛋白 (Hev b 4).乳胶中的 Hev b 6 含量比其他七种乳胶过敏原高出一千倍，具体取决于来源和/或制备程序。过敏原浓度反映在 mRNA 转录本的丰度上。当用作抗原时，乳胶血清可能会使乳胶过敏诊断测试的结果偏向于对 Hev b 6 敏感。使用乳胶血清的测试可能无法检测仅对低浓度过敏原敏感的受试者的乳胶特异性 IgE 反应性。结论使用全乳胶血清作为抗原源的乳胶过敏诊断和免疫治疗可能不是最佳的，因为其成分过敏原明显不平衡。
BackgroundHevea brasiliensislatex serum is commonly used as thein vivoandin vitroreference antigen for latex allergy diagnosis as it contains the full complement of latex allergens.ObjectiveThis study quantifies the concentrations of the significant allergens in latex serum and examines its suitability as an antigen source in latex allergy diagnosis and immunotherapy.MethodsThe serum phase was extracted from centrifuged latex that was repeatedly freeze‐thawed or glycerinated. Quantitation of latex allergens was performed by two‐site immunoenzymetric assays. The abundance of RNA transcripts of the latex allergens was estimated from the number of their clones in an Expressed Sequence Tags library.ResultsThe latex allergens, Hev b 1, 2, 3, 4, 5, 6, 7 and 13, were detected in freeze‐thawed and glycerinated latex serum at levels ranging from 75 (Hev b 6) to 0.06 nmol/mg total proteins (Hev b 4). Hev b 6 content in the latex was up to a thousand times higher than the other seven latex allergens, depending on source and/or preparation procedure. Allergen concentration was reflected in the abundance of mRNA transcripts. When used as the antigen, latex serum may bias the outcome of latex allergy diagnostic tests towards sensitization to Hev b 6. Tests that make use of latex serum may fail to detect latex‐specific IgE reactivity in subjects who are sensitized only to allergens that are present at low concentrations.ConclusionLatex allergy diagnostics and immunotherapy that use whole latex serum as the antigen source may not be optimal because of the marked imbalance of its constituent allergens.