Preliminary observation of chemokine expression in patients with Stanford type A aortic dissection
Preliminary observation of chemokine expression in patients with Stanford type A aortic dissection
复制标题
斯坦福A型主动脉夹层患者趋化因子表达的初步观察
DOI：
10.1016/j.cyto.2019.154920
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发表时间：
2020-03-01
期刊：
影响因子：
3.8
通讯作者：
Wang, Dongjin
中科院分区：
文献类型：
--
作者：
Fan, Fudong;Zhou, Qing;Wang, Dongjin
斯坦福大学A型主动脉夹层（TAAD）是一种致命的心血管疾病，但炎症细胞因子与疾病发病机制的关系尚不清楚。观察不同趋化因子的变化有助于进一步探讨TAAD的病因。收集2013年10月至2014年12月期间在我院就诊的TAAD患者（TAAD组）和健康对照（HC组）的临床数据。两组受试者均采集血样。采用蛋白质芯片技术检测80种趋化因子的表达水平。采用Luminex技术检测巨噬细胞炎性蛋白1 β（MIP-1 β）、上皮中性粒细胞活化肽78（ENA-78）、白细胞介素16（IL-16）、干扰素诱导蛋白10（IP-10）和FMS样酪氨酸激酶3（Flt-3）配体的表达。用ELISA试剂盒检测骨桥蛋白（OPN）和单核细胞趋化蛋白（MCP）水平。TAAD组的平均年龄为49.9 ± 11.2岁，HC组为48.7 ± 9.9岁。76.0%的TAAD患者和72.0%的健康对照为男性。TAAD组MIP-1 β、ENA-78表达较HC组明显降低，IL-16水平较HC组明显升高，血浆OPN水平较HC组明显升高，MCP-1、MCP-2表达较HC组明显降低。经斯皮尔曼分析，血清CRP水平与这些细胞因子水平之间无相关性。ROC曲线分析显示OPN可作为TAAD诊断的指标，敏感性为0.92，特异性为0.99。我们的结果提供了一个合理的方式来关注趋化因子在了解人类TAAD的发病机制。
Stanford type A Aortic dissection (TAAD) is a deadly cardiovascular disease but the relationship between inflammatory cytokines and disease pathogenesis is still unclear. Observation of the changes of different chemokines may help to explore the etiology of TAAD much further. Clinical data was collected from TAAD patients (TAAD group) and healthy controls (HC group) in our institute between October 2013 and December 2014. Blood sample was harvested from each subject of two groups. The expression levels of eighty chemokines were examined by protein array technology. Then we tested the expressions of macrophage inflammatory protein 1 beta (MIP-1 beta), epithelial neutrophil activating peptide 78 (ENA-78), interleukin 16 (1L-16), interferon inducible protein 10 (IP-10), and FMS-like tyrosine kinase 3 (Flt-3) ligand by using luminex technology. Osteopontin (OPN) and monocyte chemotaxis protein (MCP) levels were analyzed by ELISA kits. The mean age of TAAD group is 49.9 +/- 11.2 and 48.7 +/- 9.9 in HC group, respectively. 76.0% of TAAD patients and 72.0% of healthy controls were male. MIP-1 beta and ENA-78 expression in TAAD group were significantly lower than that in HC group, while significant increasing IL-16 level was found. Plasma levels of OPN in TAAD group increased remarkably compared with HC group, but MCP-1 and MCP-2 expression significantly decreased. No correlation was shown between serum CRP levels and plasma level of these cytokines by using Spearman analysis. ROC analysis showed that OPN could be indicators for TAAD diagnosis with sensitivity of 0.92 and specificity of 0.99. Our results provide a reasonable way to focus on the chemokines in understanding the pathogenesis of human TAAD.