Genomic analysis and gene structure of the plant carotenoid dioxygenase 4 family: A deeper study in Crocus sativus and its allies
Genomic analysis and gene structure of the plant carotenoid dioxygenase 4 family: A deeper study in Crocus sativus and its allies
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DOI：
10.1016/j.ygeno.2010.07.003
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发表时间：
2010-10-01
期刊：
影响因子：
4.4
通讯作者：
Gomez-Gomez, Lourdes
中科院分区：
文献类型：
--
作者：
Ahrazem, Oussama;Trapero, Almudena;Gomez-Gomez, Lourdes
质体小球靶向酶类胡萝卜素裂解双加氧酶（CCD 4）通过裂解环状类胡萝卜素的C-9-C-10和C-9 '-C-10'双键来介导挥发性C-13酮如β-紫罗兰酮的形成。在这里，我们报告的CCD 4基因组DNA区域的分离和分析番红花。不同的CCD 4等位基因已被确定：CsCCD 4a，发现有和没有内含子和CsCCD 4 b，显示存在一个独特的内含子。在其他番红花属植物中也观察到不同的CCD 4等位基因的存在。此外，CCD 4内含子的编码区内的位置与CCD 4基因从其他植物物种的比较表明，独立的增益/损失已经发生。CsCCD 4a和CsCCD 4 b的启动子区域与来自其他植物物种的CCD 4基因启动子的比较突出了参与光响应、热胁迫的顺式元件的保守性，以及分别参与昼夜节律调节和低温响应的顺式元件的缺失和独特存在。使用与β-葡萄糖醛酸酶（GUS）报告基因融合的1400个碱基对的DNA片段（P-CsCCD 4a）稳定转化的拟南芥植物对番红花CCD 4a启动子的功能表征表明，该序列足以驱动GUS在花中表达，特别是在花粉中检测到高水平。(C)2010年爱思唯尔公司All rights reserved.
The plastoglobule-targeted enzyme carotenoid cleavage dioxygenase (CCD4) mediates the formation of volatile C-13 ketones, such as beta-ionone, by cleaving the C-9-C-10 and C-9'-C-10' double bonds of cyclic carotenoids. Here, we report the isolation and analysis of CCD4 genomic DNA regions in Crocus sativus. Different CCD4 alleles have been identified: CsCCD4a which is found with and without an intron and CsCCD4b that showed the presence of a unique intron. The presence of different CCD4 alleles was also observed in other Crocus species. Furthermore, comparison of the locations of CCD4 introns within the coding region with CCD4 genes from other plant species suggests that independent gain/losses have occurred. The comparison of the promoter region of CsCCD4a and CsCCD4b with available CCD4 gene promoters from other plant species highlighted the conservation of cis-elements involved in light response, heat stress, as well as the absence and unique presence of cis-elements involved in circadian regulation and low temperature responses, respectively. Functional characterization of the Crocus sativus CCD4a promoter using Arabidopsis plants stably transformed with a DNA fragment of 1400 base pairs (P-CsCCD4a) fused to the beta-glucuronidase (GUS) reporter gene showed that this sequence was sufficient to drive GUS expression in the flower, in particular high levels were detected in pollen. (C) 2010 Elsevier Inc. All rights reserved.