COMPOSITE MOTIFS AND REPEAT SYMMETRY IN S-POMBE CENTROMERES - DIRECT ANALYSIS BY INTEGRATION OF NOTL RESTRICTION SITES
COMPOSITE MOTIFS AND REPEAT SYMMETRY IN S-POMBE CENTROMERES - DIRECT ANALYSIS BY INTEGRATION OF NOTL RESTRICTION SITES
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DOI：
10.1016/0092-8674(89)90789-7
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发表时间：
1989-06-02
期刊：
影响因子：
64.5
通讯作者：
YANAGIDA, M
中科院分区：
文献类型：
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作者：
CHIKASHIGE, Y;KINOSHITA, N;YANAGIDA, M
S.粟酒裂殖子着丝粒大而复杂。我们介绍了一种方法，使我们能够直接表征着丝粒DNA。分别含有cen 1、cen 2或cen 3的基因组DNA片段通过切割整合在靶位点上的NotI位点而制成，并部分限制用于PFG电泳中的远程作图。40 kb长的cen 1由两个反向的. apprx组成。10 kb基序，每个基序包含着丝粒元件DG和DH，两侧是中心区域。在cen 2中，三个基序与侧翼结构域以反向和直接方向排列，构成. apprx。70 kb长的重复区域。在cen 3中，15个拷贝的DG-DH构成了一个大于100 kb的区域。一组带有. apprx的倒置图案。15 kg的中央区可能是S.粟酒裂殖体基序似乎在染色体稳定性和分离中起作用。它们的作用可能是加性的，并且在基序内dg和dh的相互方向对于功能可能不是必需的。
S. pombe centromeres are large and complex. We introduced a method that enables us to characterize directly centromere DNAs. Genomic DNA fragments containing cen1, cen2, or cen3, respectively, are made by cleaving NotI sites integrated on target sites and are partially restricted for long-range mapping in PFG electrophoresis. The 40 kb long cen1 consists of two inverted .apprx. 10 kb motifs, each containing centromeric elements dg and dh, flanked by a central region. In cen2, three motifs are arranged in inverted and direct orientations with flanking domains, making up the .apprx. 70 kb long repetitious region. In cen3, .apprx. 15 copies of dg-dh constitute a region longer than 100 kb. A set of inverted motifs with an .apprx. 15 kg central region might be a prototype for the S. pombe centromeres. The motifs appear to play a role in chromosome stability and segregation. Their action may be additive, and the mutual directions of dg and dh inside a motif may not be essential for function.