Effect of topical application of raspberry ketone on dermal production of insulin-like growth factor-I in mice and on hair growth and skin elasticity in humans
Effect of topical application of raspberry ketone on dermal production of insulin-like growth factor-I in mice and on hair growth and skin elasticity in humans
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DOI：
10.1016/j.ghir.2008.01.005
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发表时间：
2008-08-01
影响因子：
1.4
通讯作者：
Nakagata, Naomi
中科院分区：
文献类型：
--
作者：
Harada, Naoaki;Okajima, Kenji;Nakagata, Naomi
感觉神经元激活后释放降钙素基因相关肽(CGRP)。我们最近报道，局部应用辣椒素可以通过激活小鼠和人类的感觉神经元增加皮肤胰岛素样生长因子-I(IGF-I)的产生，从而增加面部皮肤的弹性，促进头发生长。覆盆子酮(RK)是红树莓(Rubus Idaeus)中的一种主要芳香化合物，其结构与辣椒素相似。因此，RK可能激活感觉神经元，从而通过增加真皮IGF-I的产生来增加皮肤弹性和促进头发生长。在目前的研究中，我们在老鼠和人类身上检验了这种可能性。在高于1mU M的浓度下，RK显著增加野生型(WT)小鼠背根神经节神经元(DRG)CGRP的释放，这种增加可被香草素受体-1激活抑制剂卡萨西平完全逆转。在WT小鼠局部应用0.01%RK后30分钟，皮肤IGF-I水平增加，但在CGRP基因敲除小鼠中不增加。外用0.01%RK后4周，WT小鼠毛囊真皮乳突IGF-I免疫组织化学表达增强，并促进毛发再生。将0.01%RK局部应用于头皮和面部皮肤，在使用后5个月，50.0%的脱发患者(n=10)的头发生长得到促进，5名女性在使用后2周，面颊皮肤弹性增加(p<；0.04)。这些观察结果强烈表明，RK可能通过激活感觉神经元增加真皮IGF-I的产生，从而促进头发生长和增加皮肤弹性。(C)2008爱思唯尔有限公司。保留所有权利。
Sensory neurons release calcitonin gene-related peptide (CGRP) on activation. We recently reported that topical application of capsaicin increases facial skin elasticity and promotes hair growth by increasing dermal insulin-like growth factor-I (IGF-I) production through activation of sensory neurons in mice and humans. Raspberry ketone (RK), a major aromatic compound contained in red raspberries (Rubus idaeus), has a structure similar to that of capsaicin. Thus, it is possible that RK activates sensory neurons, thereby increasing skin elasticity and promoting hair growth by increasing dermal IGF-I production. In the present study, we examined this possibility in mice and humans. RK, at concentrations higher than 1 mu M, significantly increased CGRP release from dorsal root ganglion neurons (DRG) isolated from wild-type (WT) mice and this increase was completely reversed by capsazepine, an inhibitor of vanilloid receptor-1 activation. Topical application of 0.01% RK increased dermal IGF-I levels at 30 min after application in WT mice, but not in CGRP-knockout mice. Topical application of 0.01% RK increased immunohistochemical expression of IGF-I at dermal papillae in hair follicles and promoted hair re-growth in WT mice at 4 weeks after the application. When applied topically to the scalp and facial skin, 0.01% RK promoted hair growth in 50.0% of humans with alopecia (n = 10) at 5 months after application and increased cheek skin elasticity at 2 weeks after application in 5 females (p < 0.04). These observations strongly suggest that RK might increase dermal IGF-I production through sensory neuron activation, thereby promoting hair growth and increasing skin elasticity. (c) 2008 Elsevier Ltd. All rights reserved.