Influence of the regulatory protein RsmA on cellular functions in Pseudomonas aeurginosa PAO1, as revealed by transcriptome analysis
Influence of the regulatory protein RsmA on cellular functions in Pseudomonas aeurginosa PAO1, as revealed by transcriptome analysis
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DOI：
10.1099/mic.0.28324-0
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发表时间：
2006-02-01
期刊：
影响因子：
2.8
通讯作者：
O'Gara, F
中科院分区：
文献类型：
--
作者：
Burrowes, E;Baysse, C;O'Gara, F
RsmA是铜绿假单胞菌中的转录后调节蛋白，其与小的非编码调节RNA分子RsmB（RsmZ）串联工作，以调节几种毒力相关基因的表达，包括N-酰基-高丝氨酸内酯合酶基因lasl和rhll，以及氰化氢和鼠李糖脂生物合成操纵子。虽然这些直接RsmA调节的目标已经确定，RsmA对细胞活动的全面影响尚未了解。为了解决这个问题，比较了铜绿假单胞菌PAO 1和同基因rsmA突变体的转录组谱。RsmA的丧失改变了参与多种对毒力重要的途径和系统的基因的表达，包括铁的获得、假单胞菌喹诺酮信号（POS）的生物合成、多药外排泵的形成和运动性。并非所有这些影响都可以通过RsmA的既定调节作用来解释。因此，这项研究提供了第一步，以确定更多的基因下RsmA的转录后控制铜绿假单胞菌和更全面的了解RsmA对细胞功能的影响。
RsmA is a posttranscriptional regulatory protein in Pseudomonas aeruginosa that works in tandem with a small non-coding regulatory RNA molecule, RsmB (RsmZ), to regulate the expression of several virulence-related genes, including the N-acyl-homoserine lactone synthase genes lasl and rhll, and the hydrogen cyanide and rhamnolipid biosynthetic operons. Although these targets of direct RsmA regulation have been identified, the full impact of RsmA on cellular activities is not as yet understood. To address this issue the transcriptome profiles of P. aeruginosa PAO1 and an isogenic rsmA mutant were compared. Loss of RsmA altered the expression of genes involved in a variety of pathways and systems important for virulence, including iron acquisition, biosynthesis of the Pseudomonas quinolone signal (POS), the formation of multidrug efflux pumps, and motility. Not all of these effects can be explained through the established regulatory roles of RsmA. This study thus provides both a first step towards the identification of further genes under RsmA posttranscriptional control in P. aeruginosa and a fuller understanding of the broader impact of RsmA on cellular functions.