Amino acid sequence studies on the alpha chain of human fibrinogen. Characterization of 11 cyanogen bromide fragments.
Amino acid sequence studies on the alpha chain of human fibrinogen. Characterization of 11 cyanogen bromide fragments.
复制标题
人纤维蛋白原α链的氨基酸序列研究。
DOI：
10.1021/bi00627a028
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发表时间：
1977
期刊：
影响因子：
2.9
通讯作者：
T. Takagi
中科院分区：
文献类型：
--
作者：
R. Doolittle;K. Cassman;B. Cottrell;S. Friezner;J. Hucko;T. Takagi
人纤维蛋白原α链由600+/-25个氨基酸残基组成，其中10-11个为蛋氨酸。在这方面，我们已经鉴定和鉴定了11个溴化氰多肽片段，分别为2、3、26、28、28、37、51、56、60+/-5、+/-5和260+/-20个残基。其中五个的序列和六分之一的序列之前已经被报道过。我们现在报告其中另一个片段(56个残基)的完整氨基酸序列，其他四个片段的部分序列，以及最大片段的初步特征。在一项配套研究中(杜利特尔，R.F.，卡斯曼，K.G.，Cottrell，B.A.，Friezner，S.J.和Takagi，T.(1977)，生物化学16(本期后续论文))，我们从纤维蛋白原的血浆消化中获得了关键的重叠序列，这使得除了一个之外的所有这些溴化氰多肽都可以被有序排列。其中一些新报道的片段的序列揭示了阿尔法链的内部同源性，以及与贝塔链和伽马链的相应部分的结构相似。
The alpha chain of human fibrinogen consists of 600 +/- 25 amino acid residues, 10-11 of which are methionines. In this regard, we have identified and characterized 11 cyanogen bromide peptide fragments of 2, 3, 26, 28, 28, 37, 51, 56, 60 +/- 5, 64 +/- 5, and 260 +/- 20 residues, respectively. The sequences of five of these and a portion of a sixth have been reported previously. We now report the complete amino acid sequences of another of these fragments (56 residues), partial sequences for four others, and a preliminary characterization of the largest fragment. In a companion study (Doolittle, R. F., Cassman, K. G., Cottrell, B. A., Friezner, S. J., and Takagi, T. (1977), Biochemistry 16 (following paper in this issue)), we have obtained key overlap sequences from plasmic digests of fibrinogen which allow all but one of these cyanogen bromide peptides to be arranged in order. The sequences of some of these newly reported fragments have revealed an internal homology in the alpha chain, as well as structural similarities to the corresponding portions of the beta and gamma chains.