A comparison between cultivated and conventional limbal stem cell transplantation for Stevens-Johnson syndrome
A comparison between cultivated and conventional limbal stem cell transplantation for Stevens-Johnson syndrome
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DOI：
10.1016/j.ajo.2006.07.050
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发表时间：
2007-01-01
影响因子：
4.2
通讯作者：
Kinoshita, Shigeru
中科院分区：
文献类型：
--
作者：
Ang, Leonard P. K.;Sotozono, Chie;Kinoshita, Shigeru
目得：比较培养和传统角膜缘干细胞移植（LSCT）在Stevens-Johnson综合征（SJS）患者中炎症的消退和长期结果。设计：干预性病例报告。方法：一名32岁的SJS和双侧角膜缘干细胞完全缺乏的男性接受了右眼培养LSCT，三周后在左眼接受了传统LSCT。术后用药包括地塞米松0.1%和氧氟沙星0.3%眼药水和逐渐减量的全身皮质类固醇、环孢素和环磷酰胺。泪液样本收集和分析白细胞介素（IL）8 level.Results：培养LSCT后48小时，与传统LSCT后3周相比，完全角膜上皮化。眼部炎症和IL-8水平下降更迅速的眼睛培养LSCT。四年后手术，更严重的角膜瘢痕和混浊，注意到在传统的LSCT eye.Conclusions：培养LSCT导致更好的临床效果和视力，与传统的LSCT相比，基质瘢痕较少。
PURPOSE: To compare the resolution of inflammation and long-term results of cultivated and conventional limbal stem cell transplantation (LSCT) in a patient with Stevens-Johnson syndrome (SJS).DESIGN: Interventional case report.METHODS: A 32,year-old man with SJS and bilateral total limbal stem cell deficiency underwent cultivated LSCT in the right eye, followed by conventional LSCT in the left eye three weeks later. The postoperative medication included dexamethasone 0.1% and ofloxacin 0.3% eye, drops and a tapering dose of systemic corticosteroid, cyclosporine, and cyclophosphamide. Tear samples were collected and analyzed for interleukin (IL) 8 levels.RESULTS: Complete corneal epithelialization was achieved 48 hours after cultivated LSCT, compared with three weeks after conventional LSCT. Ocular inflammation and IL-8 levels decreased more rapidly in the eye with cultivated LSCT. Four years after surgery, more severe corneal scarring and opacification were noted in the conventional LSCT eye.CONCLUSIONS: Cultivated LSCT resulted in a better clinical result and vision, with less stromal scarring compared with conventional LSCT.