APPLIED PHYSICS Nuclear magnetic resonance detection and spectroscopy of single proteins using quantum logic
APPLIED PHYSICS Nuclear magnetic resonance detection and spectroscopy of single proteins using quantum logic
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DOI：
10.1126/science.aad8022
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发表时间：
2016-02-19
期刊：
影响因子：
56.9
通讯作者：
Lukin, M. D.
中科院分区：
文献类型：
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作者：
Lovchinsky, I.;Sushkov, A. O.;Lukin, M. D.
核磁共振波谱是有机化合物和生物分子结构分析的有力工具，但通常需要宏观样本量。我们使用一个传感器，它由两个量子比特组成，对应于一个电子自旋和一个辅助的核自旋，以展示室温下对附着在钻石表面的单个泛素蛋白中的多个核物种的磁共振检测和光谱。使用量子逻辑来提高读出保真度，并使用表面处理技术来延长浅层氮空位中心的自旋相干时间，我们证明了磁场的灵敏度足以在积分后1秒内检测到单个质子自旋。这种灵敏度的提高使我们能够对单个蛋白质进行高置信度检测，并使我们能够观察揭示其化学组成信息的光谱特征。
Nuclear magnetic resonance spectroscopy is a powerful tool for the structural analysis of organic compounds and biomolecules but typically requires macroscopic sample quantities. We use a sensor, which consists of two quantum bits corresponding to an electronic spin and an ancillary nuclear spin, to demonstrate room temperaturemagnetic resonance detection and spectroscopy of multiple nuclear species within individual ubiquitin proteins attached to the diamond surface. Using quantum logic to improve readout fidelity and a surface-treatment technique to extend the spin coherence time of shallow nitrogen-vacancy centers, we demonstrate magnetic field sensitivity sufficient to detect individual proton spins within 1 second of integration. This gain in sensitivity enables high-confidence detection of individual proteins and allows us to observe spectral features that reveal information about their chemical composition.