Voltage-sensitive calcium currents are acutely increased by nerve growth factor in PC12 cells.
Voltage-sensitive calcium currents are acutely increased by nerve growth factor in PC12 cells.
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PC12 细胞中的神经生长因子会急剧增加电压敏感的钙电流。
DOI：
10.1152/jn.1999.82.6.2847
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发表时间：
1999
影响因子：
2.5
通讯作者：
Guroff,G
中科院分区：
文献类型：
--
作者：
Jia,M;Li,M;Liu,XW;Jiang,H;Nelson,PG;Guroff,G
使用穿孔贴片技术记录 PC12 细胞的全细胞钙电流。电流是通过从-90 mV 的保持电势逐步去极化引起的。神经生长因子 (NGF) 在 3-5 分钟内使通过 L 型钙通道的钙电流增加 > 75%。这种增加可通过 K-252a、硝苯地平以及激酶 C 的抑制或下调来抑制。脑源性神经营养因子 (BDNF) 也可增加钙电流，但程度较小。因此，钙电流的增加可能与高亲和力或低亲和力神经生长因子受体的激活有关。突触前钙摄取的增加似乎是神经营养素对神经递质释放的短期作用的关键因素，从而导致长期增强。此外，钙摄取的控制可能是神经营养素对神经元存活和神经元保护的长期作用的重要因素。目前的数据表明，PC12 细胞可能是研究神经营养素对钙吸收影响的有用模型。
Whole cell calcium currents were recorded from PC12 cells with the perforated patch technique. Currents were evoked by step depolarization from a holding potential of −90 mV. Nerve growth factor (NGF) increased calcium currents through L-type calcium channels by >75% within 3–5 min. This increase was inhibited by K-252a, by nifedipine, and by inhibition or down-regulation of kinase C. Brain-derived neurotrophic factor (BDNF) also increased calcium current, but to a smaller extent. Thus increases in calcium current can be linked to activation of either the high- or the low-affinity nerve growth factor receptor. Increases in presynaptic calcium uptake appear to be a crucial element in the short-term actions of the neurotrophins on neurotransmitter release leading to long-term potentiation. Also, the control of calcium uptake is likely to be an important factor in the long-term actions of the neurotrophins on neuronal survival and neuronal protection. The present data indicate that the PC12 cell may be a useful model for studying the effect of the neurotrophins on calcium uptake.