Evaluation of microbial profile in dental unit waterlines and assessment of antimicrobial efficacy of two treating agents.

Evaluation of microbial profile in dental unit waterlines and assessment of antimicrobial efficacy of two treating agents.
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评估牙科治疗台水管中的微生物特征以及评估两种治疗剂的抗菌功效。

DOI:
10.17796/jcpd.37.4.l6851n9g01223450
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发表时间:
2013
期刊:
The Journal of clinical pediatric dentistry
影响因子:
--
通讯作者:
N. Reddy
N. Reddy
中科院分区:
--
文献类型:
--
作者:
J. Mungara;NC Dilna;E. Joseph;N. Reddy

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目的 牙科综合治疗机中用于冷却和冲洗高速和低速手机、空气/水注射器和洁牙器的水的质量相当重要。本研究的目的是对不同牙科专科诊所牙科综合治疗台水管中采集的水样进行微生物计数和鉴定,并了解两种处理剂对牙科综合治疗台水管消毒的效果。 研究设计 样本包括来自不同专业牙科诊所的70个牙科综合治疗台水管,检查其微生物污染。从这些牙科单元中随机选择40个单元,并分为两组,每组20个。A组用0.2%葡萄糖酸氯己定溶液在20个牙科综合治疗台中进行治疗,B组用10%聚维酮碘溶液在20个牙科综合治疗台中进行治疗,并评估微生物水平的降低。随机抽取5个牙科综合治疗台,使用矿泉水、无菌蒸馏水、新鲜自来水作为牙科综合治疗台储液瓶中的水源,检查微生物污染情况。同样,从试验组中,通过分析在3、5和7天间隔收集的水样,检查每组中的5只处理剂的效力持续一周。 结果 鉴定出的微生物大多数为革兰氏阴性菌,其中假单胞菌占总分离株的98.59%。使用0.2%洗必泰和10%聚维酮碘消毒剂对减少微生物污染非常有效,10%聚维酮碘的有效率更高(97.13%),并在3天内保持活性,到第7天逐渐失效。从不同出水口(如手柄出口、空气水注射器出口、洁牙机管路)采集的水样的微生物污染无显著差异。 结论 为了继续保持牙科综合治疗台水管的无菌性,并完成牙科诊所采用的感染控制措施,适当的消毒剂,如每天使用0.2%氯己定或每三天使用10%聚维酮碘,间歇性地保持牙科综合治疗台水管的无菌性,必须有良好的水源和有效的消毒剂。
OBJECTIVE The quality of water in a dental unit used for cooling and flushing the high and low speed handpiece, air/water syringes and the scalers is of considerable importance. The present study was carried out to enumerate and identify the microorganisms present in water samples collected from dental unit waterlines of different dental specialty clinics and to find out the efficacy of two treating agents in disinfecting dental unit waterlines. STUDY DESIGN Sample included 70 dental unit waterlines from different speciality dental clinics which were checked for microbial contamination. From these dental units 40 units were randomly selected and divided into two groups of 20 each. Group A, treatment was done in 20 dental units with 0.2% Chlorhexidine gluconate solution and Group B, treatment was done in 20 dental units with 10% Povidone iodine solution and the reduction in the microbial levels were assessed. Five dental units were randomly selected and checked the microbial contamination using mineral water sterile distilled water fresh tap water as a water source in the dental unit reservoir bottles. Also from the test group, five from each group were checked for the duration of efficacy of treating agent for one week by analyzing the water samples collected on 3,5 and 7 day intervals. RESULTS Most of the identified microorganisms are Gram negative and pseudomonas predominating up to 98.59% of the total isolates. Usage of disinfectants 0.2% Chlorhexidine and 10% Povidone Iodine were found to be very effective in reducing the microbial contamination and 10% Povidone iodine was found to be more efficient (97.13%) and active for a period of 3 days and gradually loosing its efficacy by 7th day. No significant difference were found in microbial contamination of water samples collected from different water outlets such as handpiece outlets, air water syringe outlets, scaler lines. CONCLUSION To continue maintaining the sterility of the Dental unit waterlines and to complete the infection control measures adopted in the dental clinics, suitable disinfectants like 0.2% Chlorhexidine on daily basis or 10% Povidone iodine on every 3rd day basis intermittently maintain the sterility of dental unit waterlines it is essential to have a good water source and an effective disinfectant.
PVDF 管对牙科综合治疗台水线中生物膜形成的影响。
DOI: --
发表时间: --
期刊: Dental Materials (in press)
影响因子: --
作者:
Yabune T;Imazato S;et al.
通讯作者: et al.