Activated leukocytes adsorbed on the surface of an extracorporeal circuit.
Activated leukocytes adsorbed on the surface of an extracorporeal circuit.
复制标题
活化的白细胞吸附在体外回路的表面。
DOI：
10.1046/j.1525-1594.2003.07050.x
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发表时间：
2003
影响因子：
2.4
通讯作者：
M. Sunamori
中科院分区：
文献类型：
--
作者：
N. Tabuchi;A. Shibamiya;T. Koyama;T. Fukuda;Willem van Oeveren Wv;M. Sunamori
体外回路的生物相容性主要通过循环中的血液采样来研究。然而，一部分活化的白细胞通过粘附在回路上而离开循环，并可能影响回路的生物相容性。为了揭示这些特征，我们通过乙二胺四乙酸 (EDTA)-HEPES 缓冲液清洗，从用于 6 名患者的回路中洗脱了吸附的白细胞。收集了 1.3 x 109 个白细胞，占循环白细胞的 5% 至 10%。大多数是中性粒细胞，其表达的 CD11b 抗原与体外循环期间循环中的中性粒细胞一样高。吸附的单核细胞表达的组织因子高于循环中的单核细胞（P < 0.05）。 EDTA 的洗脱过程本身仅诱导白细胞的最小激活。这些结果表明体外回路在使用过程中吸附大量活化的中性粒细胞和少量高度血栓形成的单核细胞。尽管我们的数据有限，但这种洗脱程序对于研究体外回路的生物相容性似乎很有用。
Biocompatibility of extracorporeal circuits has mostly been investigated by sampling blood in circulation. However, a proportion of activated leukocytes leave circulation by sticking to the circuits, and might affect the circuit biocompatibility. To reveal these characteristics, we eluted the adsorbed leukocytes from circuits used for 6 patients by washing with ethylenediaminetetraacetic acid (EDTA)-HEPES buffer. 1.3 x 109 leukocytes, representing 5 to 10% of the circulating leukocytes, were collected. Most were neutrophils expressing CD11b antigens as high as those in circulation during cardiopulmonary bypass. Adsorbed monocytes expressed tissue factor higher than those in circulation (P < 0.05). An elution procedure by EDTA itself induced only a minimal activation of the leukocytes. These results indicate that extracorporeal circuits adsorb a large number of activated neutrophils and a small number of highly thrombogenic monocytes during their use. Although our data is limited, this elution procedure appears useful to investigate the biocompatibility of extracorporeal circuits.
影响因子：
20.3
作者：
Ostrovsky, L;King, AJ;Kubes, P
通讯作者：
Kubes, P