The effects of stress on DNA repair capacity
The effects of stress on DNA repair capacity
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DOI：
10.1080/08870440008405589
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发表时间：
2000-01-01
影响因子：
3.3
通讯作者：
Baum, A
中科院分区：
文献类型：
--
作者：
Forlenza, MJ;Latimer, JJ;Baum, A
研究表明，与低度痛苦患者和健康对照相比，高度痛苦患者的淋巴细胞 DNA 修复减少。此外，修复缺陷与癌症风险增加有关。使用学术压力模型，我们假设与压力较低的时期相比，学生在紧张的考试期间会表现出较低水平的核苷酸切除修复 (NER)。参与者是 19 名健康的研究生水平的学生。使用非计划 DNA 合成 (UDS) 测定和载玻片放射自显影术测量淋巴细胞中的 NER。与预测相反，在控制修复的背景差异的情况下，相对于较低应力时期，较高应力时期的 NER 平均值显着增加。此外，在较高压力时期，淋巴细胞对内源性损伤的修复能力显着增强。压力似乎可以直接促进 DNA 修复。此外，压力可能会通过增加 DNA 损伤来间接促进 DNA 修复。
Research has shown that lymphocytes of high-distress patients have reduced DNA repair relative to that of low-distress patients and healthy controls. Furthermore, deficits in repair are associated with an increased risk of cancer. Using and academic stress model, we hypothesized that students would exhibit lower levels of Nucleotide Excision Repair (NER) during a stressful exam period when compared to a lower stress period. Participants were 19 healthy graduate level students. NER was measured in lymphocytes using the unscheduled DNA synthesis (UDS) assay with slide autoradiography. Contrary to prediction, mean values for NER significantly increased during the higher stress period relative to the lower stress period controlling for background differences in repair. Furthermore, lymphocytes had significantly increased repair of endogenous damage during the higher stress period. Stress appears to directly increase DNA repair. Additionally, stress may increase DNA repair indirectly by increasing damage to DNA.