The effect of induced biphasic pulsed currents on re-epithelialization of a novel wound healing model
The effect of induced biphasic pulsed currents on re-epithelialization of a novel wound healing model
复制标题
DOI：
10.1002/bem.20267
复制
发表时间：
2007-01-01
影响因子：
1.9
通讯作者：
MacNeill, Shelia
中科院分区：
文献类型：
--
作者：
Bullock, Anthony J.;Barker, Anthony T.;MacNeill, Shelia
角质形成细胞的协调迁移对于皮肤伤口愈合至关重要；角质形成细胞无法迁移到伤口中会导致慢性不愈合伤口。角质形成细胞迁移可能受到施加的电场的影响。我们的目的是研究是否可以通过施加感应双相脉冲电场来加速角质形成细胞迁移。为此，我们开发了两种体外生物系统模型：角质形成细胞集落形成模型和采用双相脉冲电流的重建皮肤伤口愈合模型。我们的体外皮肤模型能够产生类似于体内哺乳动物皮肤的跨上皮电位（TEP）。伤口愈合模型的组织学检查还表明，尽管在14天的体外实验期间没有看到基底膜重建的证据，但上皮化的发生方式与体内观察到的相似。我们发现，体外创面床中角质形成细胞集落的生长和角质形成细胞迁移并未受到频率为 0.5 Hz、100 和 200 mV/mm 的短持续时间双相脉冲电流的显着影响。
The coordinated migration of keratinocytes is crucial to cutaneous wound healing; failure of keratinocytes to migrate into a wound can lead to chronic non-healing wounds. Keratinocyte migration can be influenced by applied electrical fields. Our aim was to investigate whether keratinocyte migration could be accelerated by applying an induced biphasic pulsed electrical field. We developed two in vitro biological systems models for this purpose: a keratinocyte colony-forming model and a reconstituted skin wound healing model with biphasic pulsed currents. Our in vitro skin models were capable of generating trans-epithelial potentials (TEP) similar to in vivo mammalian skin. Histological examination of the wound healing model also indicated that re-epithelialization occurred in a similar manner to that seen in vivo, although no evidence of a reconstitution of a basement membrane was seen during the 14 days in vitro experimental period. We found that growth of keratinocyte colonies and keratinocyte migration in an in vitro wound bed were not significantly affected by induced short duration biphasic pulsed currents at a frequency of 0.5 Hz of 100 and 200 mV/mm.