Subtype specificity of anti-HBs antibodies produced by human B-cell lines isolated from normal individuals vaccinated with recombinant hepatitis B vaccine
Subtype specificity of anti-HBs antibodies produced by human B-cell lines isolated from normal individuals vaccinated with recombinant hepatitis B vaccine
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DOI：
10.1016/s0264-410x(02)00116-0
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发表时间：
2002-05-22
期刊：
影响因子：
5.5
通讯作者：
Shokri, F
中科院分区：
文献类型：
--
作者：
Shokrgozar, MA;Shokri, F
B型肝炎病毒表面抗原（HBsAg）由B型肝炎病毒（HBV）所有亚型共有的免疫显性决定簇和4个主要亚型决定簇组成。已经在血清水平对HBsAg的人抗体应答的亚型特异性进行了部分体内研究。然而，在细胞水平上没有全面的数据。本研究采用EB病毒转化法和有限稀释法，从34例重组乙型肝炎B疫苗（HBsAg/adw）免疫应答良好的成年人中建立了大量分泌抗-HBs抗体的B细胞株。用夹心ELISA和免疫印迹法检测了222份B细胞系的特异性，其中216份（97.3%）为抗a，5份（2.3%）为抗d，1份（0.4%）为抗w。抗-HBs抗体以IgG型为主，属IgG 1亚类。这些尚未在人体细胞水平上进行广泛研究的发现证实并扩展了先前在小鼠中获得的间接结果，并进一步证明了HBsAg的“a”决定簇在人体抗体应答中的免疫显性作用。(C)2002爱思唯尔科技有限公司。保留所有权利。
Hepatitis B surface antigen (HBsAg) constitutes of an immunodominant determinant common to all subtypes of hepatitis B virus (HBV) and four major subtypic determinants. Subtype specificity of the human antibody response to HBsAg has already been partially studied in vivo at serum level. No comprehensive data, however, is available at the cellular level. In this study, the methods of Epstein-Barr virus (EBV) transformation and limiting dilution assay (LDA) were used to establish a large number of B-cell lines secreting anti-HBs antibody from 34 adult individuals who were good-responders to the recombinant hepatitis B vaccine (HBsAg/adw). Specificity of 222 B-cell lines was assayed by sandwich ELISA and immunoblotting, of which 216 samples (97.3%) were identified to be anti-a, 5 samples (2.3%) as anti-d and one sample (0.4%) as anti-w. The isotype of most of the anti-HBs antibodies was IgG and belonged to the IgGl subclass. These findings which have not already been extensively investigated at the cellular level in human confirm and extend previous circumstantial results achieved in mouse and further prove the immunodominant role of the "a" determinant of HBsAg in antibody response in human. (C) 2002 Elsevier Science Ltd. All rights reserved.