CORNEAL CELL MATRIX INTERACTIONS - TYPE-VI COLLAGEN PROMOTES ADHESION AND SPREADING OF CORNEAL FIBROBLASTS
CORNEAL CELL MATRIX INTERACTIONS - TYPE-VI COLLAGEN PROMOTES ADHESION AND SPREADING OF CORNEAL FIBROBLASTS
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DOI：
10.1016/0014-4827(92)90200-r
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发表时间：
1992-06-01
影响因子：
3.7
通讯作者：
BIRK, DE
中科院分区：
文献类型：
--
作者：
DOANE, KJ;YANG, G;BIRK, DE
VI 型胶原蛋白是一种非原纤维胶原蛋白，以网络形式存在于鸡的次生基质中。 VI 型胶原蛋白在鸡角膜基质和其他系统中的免疫定位表明，VI 型胶原蛋白与细胞相关以及横纹原纤维之间存在。我们假设 VI 型胶原蛋白可能在细胞-基质相互作用中发挥作用，这对角膜发育很重要。为了检验这种可能性，我们分离并表征了牛角膜 VI 型胶原蛋白，并确定从角膜分离的 VI 型胶原蛋白的链组成和形态与从其他来源分离的胶原蛋白相似。 VI型胶原蛋白的组织形式定位于细丝，在原纤维周围形成网络并靠近角膜成纤维细胞。然后，我们分析了与次级基质中存在的其他胶原蛋白相比，VI 型胶原蛋白的相对附着和扩散，发现尽管角膜成纤维细胞与 VI 型和 I 型胶原蛋白的附着同样好，但细胞在 VI 型胶原蛋白上的扩散程度要大得多。尽管角膜成纤维细胞确实具有 RGD 依赖性受体，该受体在粘附纤连蛋白期间发挥作用，但与 VI 型胶原的附着是不依赖于 RGD 的，除非该分子变性。用可溶性 RGD 肽阻断 RGD 依赖性受体不会导致附着或扩散发生变化。这些数据表明 VI 型胶原蛋白在角膜基质发育过程中细胞-基质相互作用中的作用。
Type VI collagen is a nonfibrillar collagen present as a network throughout the chick secondary stroma. Immunolocalization of type VI collagen both in the chick corneal stroma and in other systems demonstrates that type VI collagen is present associated with cells and between striated fibrils. We hypothesize that type VI collagen may function in cell—matrix interactions important in corneal development. To examine this possibility, we have isolated and characterized bovine corneal type VI collagen and determined that the chain composition and morphology of type VI collagen isolated from cornea is similar to that isolated from other sources. The tissue form of type VI collagen was localized to filaments forming a network around fibrils and close to corneal fibroblasts. We then analyzed relative attachment and spreading on type VI collagen as compared to the other collagens present in the secondary stroma, and found that although corneal fibroblasts attach equally well to type VI and type I collagen, cells spread to a much greater extent on type VI collagen. Although corneal fibroblasts do have an RGD-dependent receptor which functions during adhesion to fibronectin, attachment to type VI collagen is RGD-independent unless the molecule is denatured. Blocking of the RGD-dependent receptor with soluble RGD peptides results in no change in attachment or spreading. These data imply a role for type VI collagen in cell-matrix interactions during corneal stroma development.