Seminal plasma peptides may determine maternal immune response that alters success or failure of pregnancy in the abortion-prone CBAxDBA/2 model
Seminal plasma peptides may determine maternal immune response that alters success or failure of pregnancy in the abortion-prone CBAxDBA/2 model
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DOI：
10.1016/j.jri.2013.03.006
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发表时间：
2013-09-01
影响因子：
3.4
通讯作者：
Chaouat, Gerard
中科院分区：
文献类型：
--
作者：
Clark, David A.;Rahmati, Mona;Chaouat, Gerard
DBA/2 交配的 CBA/J 小鼠的自然流产（吸收）涉及交配时针对父本抗原的 Treg 细胞活性缺陷。通过 BALB/c 脾细胞而非 DBA/2 脾细胞对雌性 CBA/J 进行预免疫，可防止 CBAxDBA/2 交配后随后发生流产。先前对 BALB/cxDBA/2 重组体的免疫遗传学研究表明，单个次要非 H-2(d) 肽的 H-2(d) 限制性呈递可能负责保护，而第二个独立等位基因的产物可能会促进流产。使用布雷菲德林处理的 BALB/c 和 DBA/2 脾细胞，我们发现在 BALB/c 精浆中孵育使 DBA/2 脾细胞具有保护性，而 DBA/2 精浆消除了保护。活性保护部分是
Spontaneous abortion (resorption) in the DBA/2-mated CBA/J mouse involves a deficiency in Treg cell activity against paternal antigens at the time of mating. Preimmunization of female CBA/J by BALB/c splenocytes, but not DBA/2 splenocytes, protects against subsequent abortions after a CBAxDBA/2 mating. Previous immunogenetic studies with BALB/cxDBA/2 recombinants have indicated that H-2(d)-restricted presentation of a single minor non-H-2(d) peptide might be responsible for protection, while the product of a second independent allele might promote abortions. Using brefeldin-treated BALB/c and DBA/2 splenocytes, we found that incubation in BALB/c seminal plasma rendered DBA/2 splenocytes protective and DBA/2 seminal plasma eliminated protection. The active protective moiety was