Identification of depot-specific human fat cell progenitors through distinct expression profiles and developmental gene patterns
Identification of depot-specific human fat cell progenitors through distinct expression profiles and developmental gene patterns
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DOI：
10.1152/ajpendo.00202.2006
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发表时间：
2007-01-01
影响因子：
5.1
通讯作者：
Kirkland, James L.
中科院分区：
文献类型：
--
作者：
Tchkonia, Tamara;Lenburg, Marc;Kirkland, James L.
通过不同的表达谱和发育基因模式鉴定储存库特异性人类脂肪祖细胞。Am J Physiol Endocrinol Metab 292：E298-E307，2007.首次发表于2006年9月19日; doi：10.1152/ajpendo.00202.2006。解剖学上分离的脂肪库在大小、功能和对病理状态（如代谢综合征）的贡献方面不同。我们从不同的人脂肪库中分离前脂肪细胞，以确定这种变化的基础是否部分归因于脂肪祖细胞固有特性的差异。我们发现，从腹部皮下、肠系膜和网膜脂肪库平行培养的原代前脂肪细胞的全基因组表达谱是不同的。有趣的是，内脏脂肪并不均匀。前脂肪细胞的两个主要的内脏仓库之一，肠系膜脂肪，有一个表达谱更接近皮下比网膜前脂肪细胞，其他主要的内脏仓库。从不同的脂肪库中分离的未分化前脂肪细胞中，调控早期发育的基因（包括同源异型基因）的表达存在很大差异。通过对来自另外的瘦型和肥胖型男性和女性受试者的前脂肪细胞的实时PCR分析证实了这些特征。我们通过表达端粒酶从单个腹部皮下和网膜前脂肪细胞中制备前脂肪细胞株。库特异性发育基因表达谱持续40人口doubests在这些菌株。因此，来自不同区域的人脂肪祖细胞实际上是不同的，这与作为单独的微型器官的不同脂肪库一致。
Identification of depot-specific human fat cell progenitors through distinct expression profiles and developmental gene patterns. Am J Physiol Endocrinol Metab 292:E298-E307, 2007. First published September 19, 2006; doi:10.1152/ajpendo.00202.2006.-Anatomically separate fat depots differ in size, function, and contribution to pathological states, such as the metabolic syndrome. We isolated preadipocytes from different human fat depots to determine whether the basis for this variation is partly attributable to differences in inherent properties of fat cell progenitors. We found that genome-wide expression profiles of primary preadipocytes cultured in parallel from abdominal subcutaneous, mesenteric, and omental fat depots were distinct. Interestingly, visceral fat was not homogeneous. Preadipocytes from one of the two main visceral depots, mesenteric fat, had an expression profile closer to that of subcutaneous than omental preadipocytes, the other main visceral depot. Expression of genes that regulate early development, including homeotic genes, differed extensively among undifferentiated preadipocytes isolated from different fat depots. These profiles were confirmed by real-time PCR analysis of preadipocytes from additional lean and obese male and female subjects. We made preadipocyte strains from single abdominal subcutaneous and omental preadipocytes by expressing telomerase. Depot-specific developmental gene expression profiles persisted for 40 population doublings in these strains. Thus, human fat cell progenitors from different regions are effectively distinct, consistent with different fat depots being separate mini-organs.