Characterization of oral bacteria in the tongue coating of patients with halitosis using 16S rRNA analysis
Characterization of oral bacteria in the tongue coating of patients with halitosis using 16S rRNA analysis
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DOI：
10.1080/00016357.2020.1754459
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发表时间：
2020-04-20
影响因子：
2
通讯作者：
Kawaguchi, Yoko
中科院分区：
文献类型：
--
作者：
Oshiro, Akiko;Zaitsu, Takashi;Kawaguchi, Yoko
关于舌苔与口臭之间关系的研究很多，但对舌苔细菌群落的研究很少。本研究利用16S rRNA分析鉴定了有和没有口臭的人舌苔中的细菌。选取2016 - 2017年在大学牙科医院口臭门诊就诊的40例患者（平均年龄46.1 +/- 15.8岁），根据嗅觉测试（OT）结果分为口臭组（n = 32）和非口臭组（n = 8）。通过气相色谱（GC）和Breathtron (R)仪器进行的额外测量证实，口臭组的H2S、CH3SH、（CH3）(2)S和总挥发性硫化合物（VSC）水平显著高于非口臭组。口臭组细菌多样性较高；口臭组Shannon指数中位数（四分位数）为4.46(4.21,4.67)，非口臭组为3.80（3.45,4.30）。此外，口臭组Chao-1指数中位数（四分位数）为84.0(77.2,95.0)，非口臭组为71.3（65.0,81.5）。这些细菌组成和多样性的差异可能会进一步了解口臭的原因和治疗。
There are many studies on the relationship between the tongue coating and halitosis, but few have evaluated the bacterial community present in the tongue coating. This study identified bacteria in the tongue coating in individuals with and without halitosis using 16S rRNA analysis. Forty subjects (mean age, 46.1 +/- 15.8 years) who visited the halitosis clinic at the University Dental Hospital between 2016 and 2017 were divided into halitosis (n = 32) and non-halitosis (n = 8) groups according to results from an organoleptic test (OT). Additional measurements via gas chromatography (GC) and the Breathtron (R) instrument confirmed the groupings as the H2S, CH3SH, (CH3)(2)S, and total volatile sulphur compounds (VSC) levels were significantly higher in the halitosis group than in the non-halitosis group. Bacterial diversity was higher in the halitosis group; the median (quartile) values of the Shannon index were 4.46 (4.21, 4.67) in the halitosis group and 3.80 (3.45, 4.30) in the non-halitosis group. Additionally, the median (quartile) values of the Chao-1 index were 84.0 (77.2, 95.0) in the halitosis group and 71.3 (65.0, 81.5) in the non-halitosis group. These differences in bacterial composition and diversity may further the understanding of causes and treatments for halitosis.