Identification of a novel host protein SINAL10 interacting with GP64 and its role in Bombyx mori nucleopolyhedrovirus infection
Identification of a novel host protein SINAL10 interacting with GP64 and its role in Bombyx mori nucleopolyhedrovirus infection
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与 GP64 相互作用的新型宿主蛋白 SINAL10 的鉴定及其在家蚕核多角体病毒感染中的作用
DOI：
10.1016/j.virusres.2018.02.005
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发表时间：
2018-03-02
期刊：
影响因子：
5
通讯作者：
Wu, Xiaofeng
中科院分区：
文献类型：
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作者：
Feng, Min;Kong, Xiangshuo;Wu, Xiaofeng
家蚕核型多角体病毒（BmNPV）是家蚕最重要的病原体，给丝绸业造成严重损失。在病毒感染周期中，产生了具有相同遗传内容但不同表型的芽殖病毒（BV）和闭塞衍生病毒（ODV）颗粒。在BV中特异性的包膜糖蛋白GP 64参与宿主细胞受体结合，并且足以在病毒进入期间介导膜融合。然而，与GP 64相互作用以介导BV感染的宿主细胞因子仍然是未知的。本研究构建了家蚕细胞（BmN）的cDNA文库，并利用酵母双杂交技术筛选与GP 64相互作用的宿主细胞因子。通过免疫共沉淀实验进一步证实了其中一个编码E3泛素-蛋白连接酶SINA-like 10（SINAL 10）的候选蛋白是新的GP 64结合蛋白。此外，SINAL 10的过表达显著增强病毒繁殖，相反，通过小干扰RNA沉默其表达显示出显著的抑制作用。总的来说，我们证明SINAL 10是一种新的GP 64结合蛋白，刺激BmNPV增殖。
Bombyx mori nucleopolyhedrovirus (BmNPV) is the most important pathogen of Bombyx mori, silkworm and causes severe losses in the silk industry. During the virus infectious cycle, budded virus (BVs) and occlusion derived virus (ODVs) particles, which have identical genetic content but different phenotypes, are produced. The envelope glycoprotein GP64, specific in BVs, is involved in host cell receptor binding and is sufficient to mediate membrane fusion during the viral entry. However, the host cell factors, interacting with GP64 to mediate BVs infection, are still unknown. In this study, a cDNA library of Bombyx mori cells (BmN) was constructed and yeast two-hybrid screening was used to identify the host cell factors interacting with GP64. One of the eight candidate proteins encoded the E3 ubiquitin-protein ligase SINA-like 10 (SINAL10), was further confirmed through coimmunoprecipitation assays as novel GP64 binding protein. Moreover, overexpression of SINAL10 significantly enhances viral reproduction, and conversely, silencing its expression by small interfering RNAs showed significant inhibitory effects. Collectively, we demonstrated that SINAL10 is a novel GP64-binding protein that stimulates BmNPV proliferation.