Essential role for gene profiling analysis in the authentication of human cell lines
Essential role for gene profiling analysis in the authentication of human cell lines
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DOI：
10.1111/j.1749-0774.2005.00007.x
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发表时间：
2006-02
期刊：
影响因子：
4.3
通讯作者：
Kaori Yoshino;Emi Iimura;K. Saijo;S. Iwase;K. Fukami;T. Ohno;Y. Obata;Yukio Nakamura
中科院分区：
文献类型：
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作者：
Kaori Yoshino;Emi Iimura;K. Saijo;S. Iwase;K. Fukami;T. Ohno;Y. Obata;Yukio Nakamura
培养细胞系之间的交叉污染可能导致产生错误的科学数据。因此，消除与所要求的来源不同的细胞系非常重要。种间污染可以通过各种已建立的方法检测，如核型和同工酶分析。然而，在开发技术以在分子水平上检测细胞系之间的差异之前，不可能检测种内交叉污染。近年来，短串联重复序列（STR）多态性分析已成为基因多态性分析的一种方法。通过STR多态性进行基因分析（STR分析）是识别单个细胞系的简单可靠的方法。目前由RIKEN BioResource Center的细胞工程部门提供的每个人细胞系都通过STR分析进行分析，以验证其身份。我们发现，在大约400个人类细胞系中，有10个以上的细胞系实际上与收藏中的不同细胞系相同，因此被错误识别。我们的结论是，STR分析是一个有用的和强大的方法，消除已被错误识别的交叉污染或其他原因的细胞系。因此，在已发表的研究中使用的人类细胞系的STR分析可能是未来发表的先决条件，以便可以消除细胞系错误识别的问题。
Cross-contamination between cultured cell lines can result in the generation of erroneous scientific data. Hence, it is very important to eliminate cell lines that are of an origin different from that being claimed. Inter-species contamination can be detected by various established methods, such as karyotype and isozyme analyses. However, it has been impossible to detect intraspecies cross-contamination prior to the development of technology to detect differences between cell lines at the molecular level. Recently, profiling of short tandem repeat (STR) polymorphisms has been established as a method for the analyses of gene polymorphism. Gene profiling by STR polymorphism (STR profiling) is a simple and reliable method to identify individual cell lines. Each human cell line currently provided by the Cell Engineering Division of the RIKEN BioResource Center was analyzed by STR profiling to authenticate its identity. We found that more than 10 human cell lines out of approximately 400 were in fact identical to a different cell line deposited in the collection, and therefore had been misidentified. We conclude that STR profiling is a useful and powerful method for eliminating cell lines that have been misidentified by cross-contamination or by other causes. Hence, STR profiling of human cell lines used in published research will likely be a prerequisite for publication in the future, so that the problem of misidentification of cell lines can be eliminated.