Disruption of mouse poly(A) polymerase mGLD-2 does not alter polyadenylation status in oocytes and somatic cells
Disruption of mouse poly(A) polymerase mGLD-2 does not alter polyadenylation status in oocytes and somatic cells
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DOI：
10.1016/j.bbrc.2007.09.096
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发表时间：
2007-12-07
影响因子：
3.1
通讯作者：
Baba, Tadashi
中科院分区：
文献类型：
--
作者：
Nakanishi, Tomoko;Kumagai, Satoshi;Baba, Tadashi
胞质中poly（A）尾的延伸是非洲爪蟾卵子发生和早期胚胎发生所必需的。mGLD-2是爪蟾胞质聚腺苷酸聚合酶xGLD-2的小鼠同源物。我们发现了一个协会的mGLD-2与胞质聚腺苷酸化组件，CPEB和CPSF描述Xenoplis卵母细胞。为了阐明mGLD-2在小鼠中的作用，我们通过同源重组产生mGLD-2破坏的小鼠系。尽管mGLD-2的普遍表达，但被破坏的小鼠显然是正常和健康的。此外，使用报告RNA证明mGLD-2破坏不会影响卵母细胞中poly（A）尾的延伸。与这些观察结果一致，卵母细胞成熟正常，小鼠具有生育能力。因此，MGLD-2是一种适合于足月发育和卵子发生的基因。我们的研究结果还表明，在小鼠细胞质中存在另一种来源的poly（A）聚合酶。(C)2007爱思唯尔公司All rights reserved.
The elongation of poly(A) tails in cytoplasm is essential for oogenesis and early embryogenesis in Xenopus laevis. mGLD-2 is a mouse homologue of Xenopus cytoplasmic poly(A) polymerase xGLD-2. We found an association of mGLD-2 with cytoplasmic polyadenylation components, CPEB and CPSF described in Xenoplis oocytes. To clarify the role of mGLD-2 in mouse, we produced an mGLD-2 disrupted mouse line by homologous recombination. In spite of the ubiquitous expression of mGLD-2, the disrupted mice were apparently normal and healthy. Moreover, it was demonstrated that mGLD-2 disruption did not affect the poly(A) tail elongation in oocytes using reporter RNAs. Coincide with these observations, the maturation of the oocytes was normal and the mice were fertile. Thus MGLD-2 is dispensable for full-term development and oogenesis. Our results also indicate that there is another source of cytoplasmic poly(A) polymerase in mouse. (C) 2007 Elsevier Inc. All rights reserved.